Creation of A Light-Driven Marine Vibrio Factory for Biofuel Production

• Project/Area Number: 26660168
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Sawabe Tomoo 北海道大学, 水産科学研究科(研究院), 教授 (30241376)
• Co-Investigator(Kenkyū-buntansha): HOSOKAWA MASASHI 北海道大学, 大学院水産科学研究院, 准教授 (10241374)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生可能エネルギー / 光栄養性 / 水産学 / バイオマス / 微生物 / プロテオロドプシン / カロテノイド / 代謝改変 / ゲノム

Outline of Final Research Achievements

An aim of the study is to develop light-driven marine vibrio biocatalysts in efficient biofuel production. We performed 1) gene expression of a Nonlabens sediminis proteorhdopsin gene, and 2) a cloning of a myxol synthetic gene cluster of Nonlabens ulvanivorans. We also accumulated fundamental knowledge on CRISPR/Cas system for further developments of a genome editing approach working in marine vibrio cells.

Research Products

• [Journal Article] Draft Genome Sequences of Two Vibrionaceae Species, Vibrio ponticus C121 and Photobacterium aphoticum C119, Isolated as Coral Reef Microbiota (2014)
  • Author(s): N. Al-Saari, P.M. Meirelles, S. Mino, W. Suda, K. Oshima, M. Hattori, M. Ohkuma, F.L. Thompson, B. Gomez-Gil, T. Sawabe, T. Sawabe
  • Journal Title: Genome Announc. Volume: 2 Issue: 5
  • DOI: 10.1128/genomea.01095-14
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequences of Marine Flavobacterium Nonlabens Strains NR17, NR24, NR27, NR32, NR33, and Ara13 (2014)
  • Author(s): M. Nakanishi, P. Meirelles, R. Suzuki, N. Takatani, S. Mino, W. Suda, K. Oshima, M. Hattori, M. Ohkuma, M. Hosokawa, K. Miyashita, F.L. Thompson, A. Niwa, T. Sawabe, T. Sawabe
  • Journal Title: Genome Announc. Volume: 2 Issue: 6
  • DOI: 10.1128/genomea.01165-14
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequences of Marine Flavobacterium Algibacter lectus Strains SS8 and NR4 (2014)
  • Author(s): Takatani N, Nakanishi M, Meirelles P, Mino S, Suda W, Oshima K, Hattori M, Ohkuma M, Hosokawa M, Miyashita K, Thompson FL, Niwa A, Sawabe T, Sawabe T
  • Journal Title: Genome Announc. Volume: 2 Issue: 6
  • DOI: 10.1128/genomea.01168-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draft Genome Sequence of Marine Flavobacterium Jejuia pallidilutea Strain 11shimoA1 and Pigmentation Mutants (2014)
  • Author(s): Takatani N, Nakanishi M, Meirelles P, Mino S, Suda W, Oshima K, Hattori M, Ohkuma M, Hosokawa M, Miyashita K, Thompson FL, Niwa A, Sawabe T, Sawabe T
  • Journal Title: Genome Announc. Volume: 2 Issue: 6
  • DOI: 10.1128/genomea.01236-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ビブリオ科細菌が有するCRISPR/Casシステムの特徴 (2015)
  • Author(s): 遠藤祥子・美野さやか・澤辺智雄
  • Organizer: 第67回生物工学会大会
  • Place of Presentation: 城山観光ホテル（鹿児島県、鹿児島市）
  • Year and Date: 2015-10-26
• [Presentation] 海洋細菌が有するカロテノイド合成系の体系的理解 (2014)
  • Author(s): 中西誠人・高谷直己・高峰・宮下和夫・細川雅史・大熊盛也・大島健志朗・服部正平・澤辺智雄
  • Organizer: 日本水産学会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2014-09-14 – 2014-09-17