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Study of the impact of bacteriophages on the microbiota in aquaculture environments using metagenome analysis

Research Project

Project/Area Number 26660170
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

SANO Motohiko  東京海洋大学, 学術研究院, 教授 (00372053)

Co-Investigator(Kenkyū-buntansha) 近藤 秀裕  東京海洋大学, 学術研究院, 准教授 (20314635)
Co-Investigator(Renkei-kenkyūsha) HIRONO Ikuo  東京海洋大学, 学術研究院, 教授 (00270926)
Research Collaborator SAMESHIMA Mamoru  
NOMURA Yoshinori  
NAKANE Motoyuki  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords海洋生態 / 海洋微生物 / ファージ / マリンゲノム / メタゲノム
Outline of Final Research Achievements

We tried to show the impact of bacteriophage (phage) to microbiota in shrimp aquaculture using metagenome analysis. Application of metagenome analysis using MiSeq platform to rearing water of shrimp aquaculture pond was studied at the start. The results showed that the analysis could be applied to detect the whole plankton and bacterial population including unculturable ones in the water, which have been remained as a black box. It makes us posible to monitor the dynamics of planktons and bacteria in shrimp aquaculture. To monitor phage dynamics in the environment, we isolated new phages infecting environmental bacteria such as Cytophaga-Flavobacterium group bacteria and deposited the genome sequences. Further we tried to detect phage DNA in the pond water using metagenome analysis, but we obtained a small amount of bacteriophage DNAs and the most of the sequences were remained unknown in reference virus databases. The virus sequences obtained may be of the virus infecting planktons.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] マレーシア大学サバ校ボルネオ海洋研究所(マレーシア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Borneo Marine Research Institute(マレーシア)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Complete Genome Sequence of Lytic Giant Bacteriophage pT24 Infecting Tenacibaculum sp. Isolated from Shrimp Culture Pond2017

    • Author(s)
      Ho Viet Khoa, Yuki Midorikawa, Tsubasa Uchino, Toshihiro Nakai, Goshi Kato, Hidehiro Kondo, Ikuo Hirono, Matthura Labaiden, Sataporn Direkbusarakom, Motohiko Sano
    • Journal Title

      Genome Announcement

      Volume: -

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation and Characterization of Large Marine Bacteriophage (Myoviridae), VhKM4 Infecting Vibrio harveyi2017

    • Author(s)
      Tamrin M. Lal , Motohiko Sano, Julian Ransangan
    • Journal Title

      Journal of Aquatic Animal Health

      Volume: 29 Issue: 1 Pages: 26-30

    • DOI

      10.1080/08997659.2016.1249578

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete genome sequence of a giant Vibrio phage ValKK3 infecting Vibrio alginolyticus2016

    • Author(s)
      Tamrin M. Lal, Motohiko Sano, Kishio Hatai, Julian Ransangan
    • Journal Title

      Genomics Data

      Volume: 8 Pages: 37-38

    • DOI

      10.1016/j.gdata.2016.03.002

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete genome sequence analysis of two Pseudomonas plecoglossicida phages, potential therapeutic agents2015

    • Author(s)
      Kawato Y, Yasuike M, Nakamura Y, Shigenobu Y, Fujiwara A, Sano M, Nakai T
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81 Issue: 3 Pages: 874-881

    • DOI

      10.1128/aem.03038-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Metagenome monitoring of biological environment in shrimp culture pond2017

    • Author(s)
      Yuki Midorikawa, Tsubasa Uchino, Yoshinori Nomura, Motoyuki Nakane, Mamoru Sameshima, Hidehiro Kondo, Ikuo Hirono, Matthura Labaiden, Sataporn Direkbusarakom, Goshi Kato and Motohiko Sano
    • Organizer
      JSFS International Symposium on “Fisheries Science for the Future Generations
    • Place of Presentation
      東京
    • Year and Date
      2017-09-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] APPLICATION OF METAGENOMIC ANALYSIS TO BIOLOGICAL ENVIRONMENT MONITORING OF SHRIMP CULTURE POND2017

    • Author(s)
      Yuki Midorikawa, Tsubasa Uchino, Yoshinori Nomura, Motoyuki Nakane, Mamoru Sameshima, Hidehiro Kondo, Ikuo Hirono, Matthura Labaiden, Sataporn Direkbusarakom, Goshi Kato and Motohiko Sano
    • Organizer
      10th Symposium on Diseases in Asian Aquaculture
    • Place of Presentation
      バリ、インドネシア
    • Year and Date
      2017-08-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メタゲノム解析によるクルマエビ養殖池の生物環境 モニタリングへ向けた基礎的研究2017

    • Author(s)
      翠川優希・内野翼・野村昌功・郡司掛博昭・ 中根基行・鮫島守・ 加藤豪司・近藤秀裕・佐野元彦
    • Organizer
      平成29年度日本水産学会春大会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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