| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We examined transcriptional dynamics of genes of cyanobacteria Microcystis aeruginosa and its pahge during infection through RNA-seq and in silico analysis. Phage gene dynamics showed three expression classes like other lytic dsDNA phages including marine cyanophages; early (host-takeover), middle (replication) and late genes (virion morphogenesis). The early genes are concentrated in a single ~5.8 kb window on the phage genome spanning 10 open-reading frames. Almost all host genes (99%) did not show significant change in expression throughout infection. Bacterial RNA polymerase (sigma70)-recognition sequences were also found in the upstream region of middle and late genes, but not the phage-specific motifs. Our findings suggested that Ma-LMM01 phage archived sequential three expression patterns with no change in host promoter activity unlike known T4-like phages.
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