Studies on mechanisms of viral takeover of its cyanobacterial host for application platform of a next generation fermentation system
| Project/Area Number |
26660171
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
左子 芳彦 京都大学, 農学研究科, 教授 (60153970)
澤山 茂樹 京都大学, 農学研究科, 教授 (80357178)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | シアノファージ / ラン藻 / ミクロキスティス / トランスクリプトーム / 光発酵 |
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| Outline of Final Research Achievements |
We examined transcriptional dynamics of genes of cyanobacteria Microcystis aeruginosa and its pahge during infection through RNA-seq and in silico analysis. Phage gene dynamics showed three expression classes like other lytic dsDNA phages including marine cyanophages; early (host-takeover), middle (replication) and late genes (virion morphogenesis). The early genes are concentrated in a single ~5.8 kb window on the phage genome spanning 10 open-reading frames. Almost all host genes (99%) did not show significant change in expression throughout infection. Bacterial RNA polymerase (sigma70)-recognition sequences were also found in the upstream region of middle and late genes, but not the phage-specific motifs. Our findings suggested that Ma-LMM01 phage archived sequential three expression patterns with no change in host promoter activity unlike known T4-like phages.
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Report
(4 results)
Research Products
(6 results)
