Evolutionary analysis of deep-sea microorganisms incomplete in nuclear membrane structure inhabiting the Wednesday Seamount
| Project/Area Number |
26660176
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Tokyo Metropolitan Industrial Technology Research Institute (2016-2017)
Tokyo Medical University (2014-2015) |
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Principal Investigator |
Hachiya Naomi 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 主任研究員 (30408075)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | レーザーマイクロダイセクション法 / 深海微生物 / 蛋白質可溶化 / レーザーマイクロダイセクション / ミトコンドリア / 電子顕微鏡 / 微細構造観察 / 深海 |
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| Outline of Final Research Achievements |
Since no organisms linking prokaryotes and eukaryotes have been found, it is likely that they have already been culled in the process of evolution or live in places difficult to collect. In this research, we anticipate the possibility of the latter, find out the organisms using microorganisms collected from the deep-sea floor of the Izu-Ogasawara offshore Seamount. Through comprehensive screening by transmission electron microscopy, we found the possibility that microorganisms attached to deep sea weed beetle collected are such organisms. Furthermore, for the experimental analysis, we have developed an advanced system of the laser microdissection system (Advanced-LMD) which easily cut microscopic region of less than 1 μm in diameter. In addition, we constructed a system to recover a large number of samples at once by the capillary phenomenon of the glass capillary.
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Comprehensive proteomic profiles of mouse AApoAII amyloid fibrils provide insights into the involvement of lipoproteins in the pathology of amyloidosis2018
Author(s)
Miyahara H, Sawashita J, Ishikawa E, Yang M, Ding X, Liu Y, Hachiya N, Kametani F, Yazaki M, Mori M, Higuchi K
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Journal Title
J Proteomics
Volume: 172
Pages: 111-121
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The pathological and biochemical identification of possible seed-lesions of transmitted transthyretin amyloidosis after domino liver transplantaion.2015
Author(s)
Yoshinaga, T, Yazaki M, Sekijima Y, Kametani F, Miyashita K, Hachiya N, Tanaka T, Koaudo N, Higuchi K, Ikeda S.
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Journal Title
J Path: Clin Res 2015
Volume: 1
Issue: 2
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] アミロイド再考2015
Author(s)
八谷如美
Organizer
BMB2015ワークショップ
Place of Presentation
神戸
Year and Date
2015-12-01
Related Report
Invited
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[Presentation] 認知症克服に向けて2014
Author(s)
八谷如美
Organizer
日本生化学会第87回大会
Place of Presentation
京都
Year and Date
2014-10-15 – 2014-10-18
Related Report
Invited
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