• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of sexual reproduction in oil-producing algae for cross breeding

Research Project

Project/Area Number 26660179
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionOsaka Institute of Technology

Principal Investigator

Kawamura Koji  大阪工業大学, 工学部, 准教授 (00595613)

Co-Investigator(Renkei-kenkyūsha) Okada Shigeru  東京大学, 農学生命科学研究科, 准教授 (00224014)
Research Collaborator Ardianor  University of Palangka Raya, Faculty of Agriculture, Lecturer
Hendrik Segah  University of Palangka Raya, Faculty of Agriculture, Researcher
Gumiri Sulmin  University of Palangka Raya, International Office, Professor
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords交配育種 / 有性生殖 / 微細藻類 / バイオ燃料 / リアルタイムPCR / 減数分裂 / 炭化水素産生藻 / 遺伝資源 / 藻類バイオ燃料 / ダイレクトPCR / オイル産生藻類 / Botryococcus braunii
Outline of Final Research Achievements

Oil-producing microalgae produce oil such as lipid and hydrocarbon by utilizing solar energy and carbon dioxide (phosynthesis). Microalgal oil can become renewable and carbon-neutral next-generation energy. However, known oil-producing microalgae are not enough efficient to produce oil for mass production. Breeding method should be developed in microalgae for improving the productivity. We therefore aim to uncover the mechanisms of sexual reproduction of oli-producing microalgae for cross breeding and to search novel genetic resources in wild species and strains for materials of breeding. By genomic screening and gene expression analysis, we found an evidence of capability for sexual reproduction in an oil-producing microalga, Botryococcus braunii. We have also developed a PCR (polymerase chain reaction) based technique for detection and quantification of wild B. braunii strains from tropics to temperate regions.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Presentation (10 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] パランカラヤ大学(インドネシア)

    • Related Report
      2015 Research-status Report
  • [Presentation] 熱帯産と温帯産の炭化水素産生藻Botryococcus brauniiの温度耐性の比較2017

    • Author(s)
      西川鈴音, 平野昂太郎, Aridianor A, Segah H, Sulmin G, 岡田茂, 河村耕史
    • Organizer
      第41回日本藻類学会
    • Place of Presentation
      高知大学
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 炭化水素産生藻Botryococcus brauniiiの転写活性を有するトランスポゾンの探索2017

    • Author(s)
      原拓也, 小松原直也, 岡田茂, 河村耕史
    • Organizer
      第41回日本藻類学会
    • Place of Presentation
      高知大学
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] リアルタイムPCRを利用したオイル産生藻類Botryococcus braunii野生株の検出と定量2017

    • Author(s)
      原拓也, 平野昂太郎, Ardianor A, Segah H, Sulmin G, 岡田茂, 河村耕史
    • Organizer
      第51回日本水環境学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Application of real-time PCR assay for detection and quantification of Botryococcus braunii in a wide range of natural environments from tropics to temperate2016

    • Author(s)
      Hirano K, Hara T, Ardianor A, Segah H, Sulmin G, Kawamura K.
    • Organizer
      The 10th Algal Biomass Summit
    • Place of Presentation
      Phoenix, Arizona, USA
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Direct-PCR Method for Race Identification and Performance Evaluation of Botryococcus braunii Wild Strains Isolated from Temperate to Tropical Environments2016

    • Author(s)
      Nishikawa S, Shintani H, Adianor A, Segah H, Sulmin G, Takayama N, Komai Y, Okada S, Kawamura K.
    • Organizer
      The 6th International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of real-time PCR assay for detection and quantification of hydrocarbon-producing microalga Botryococcus braunii race B2016

    • Author(s)
      Kawamura K, Hara T, Okada S.
    • Organizer
      The 6th International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The existence of 9 meiotic genes in Botryococcus braunii and the conditions for their expression2016

    • Author(s)
      Ono Y, Kawaharada R, Okada S, Kawamura K.
    • Organizer
      The 6th International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression analysis of Squalene Synthase-like 3 (SSL3) gene in Botrycoccus braunii showa strain: Culture conditions affecting SSL3 expression2016

    • Author(s)
      Hara T, Okada S, Kawamura K.
    • Organizer
      The 6th International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-06-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微細藻類のクロレラの脂質生産を最大化するための培養条件の検討2016

    • Author(s)
      角井今日子、河村耕史、古崎康哲、松本政秀、石川宗孝
    • Organizer
      日本水環境学会
    • Place of Presentation
      アスティとくしま ASTY
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 微細藻類によるCO2排出削減技術2015

    • Author(s)
      河村耕史
    • Organizer
      日本農業気象学会近畿支部
    • Place of Presentation
      大阪工業大学うめきたナレッジセンター
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
    • Invited

URL: 

Published: 2014-04-04   Modified: 2022-02-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi