New molecular discrimination for animal species using DNA polymorphism
| Project/Area Number |
26660212
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 分子種判別 / 動物 / ミトコンドリアDNA / DNA鑑定 / 簡便・迅速・正確 / PAS検出 / 共通性と特異性 / 種特異的PCR primer / Multiplex PCR / 簡便法 / 迅速・正確 / 特異的PCR primer |
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| Outline of Final Research Achievements |
Food problems are various, contamination of the food, such as animal hair, even for such repellent foods such as problems which are allergies, religion and culture, the interest is high not only in Japan but also over the world. In these methods many problems occurred, such as the minute size, poor preservation conditions and absence of DNA for analysis. Here, we used Multiplex PCR that can analyze many samples simultaneously. In addition, printed array strip (PAS) was also used which made analysis quick, simple and cheap. We have designed specific PCR primers in each animal species (domestic, wild and pet animals), and PCR products for all animal species were obtained. So, this method was very useful for species discrimination from fresh, processed meat and food made from domestic and wild animals. Also it was useful for forensics identification of human and animals. Therefore it was possible to construct a fast, simple and high sensitive detection method in molecular discrimination.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] 鳥類の分子性判別技術に関する研究2014
Author(s)
浦崇明，野田亜矢子，南 心司，西堀正英
Organizer
日本動物遺伝育種学会第 15 回大会
Place of Presentation
独立行政法人理化学研究所
Year and Date
2014-10-30 – 2014-11-01
Related Report
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