Study of pathogenic mechanism regulated by virus-derived noncoding RNA

• Project/Area Number: 26660220
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: YOSHII Kentaro 北海道大学, (連合)獣医学研究科, 准教授 (50421988)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: フラビウイルス / 脳炎 / ノンコーディングRNA / 非翻訳領域 / ダニ媒介性脳炎ウイルス

Outline of Final Research Achievements

Flaviviruses include many clinically important zoonotic pathogens, but little is known about the pathogenic mechanism. In our previous study, we showed that the 3’-untranslated region (UTR) is involved in the pathogenicity of the strains of tick-borne encephalitis virus (TBEV). In this study, to investigate the detailed function of the 3’-UTR, we constructed recombinant TBEV with partial deletions in the variable region of the 3’-UTR. In a mouse model, the partial deletions drastically increased the virulence of the virus, with no effect on virus multiplication in mouse brain. These data suggested that the conformational structure of the variable region is associated with the pathogenicity of TBEV. These findings provide a foundation for further research to identify the pathogenic mechanisms of TBEV.

Research Products

• [Journal Article] Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles. (2016)
  • Author(s): Inagaki E, Sakai M, Hirano M, Muto M, Kobayashi S, Kariwa H, Yoshii K
  • Journal Title: Ticks Tick Borne Dis Volume: S1877-959X Issue: 5 Pages: 30039-30045
  • DOI: 10.1016/j.ttbdis.2016.03.002
  • NAID: 120006318731
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Flavivirus antagonism of type I interferon signaling reveals prolidase as a regulator of IFNAR1 maturation and expression. (2015)
  • Author(s): Lubick KJ, Robertson SJ, McNally KL, Freedman BA, Rasmussen AL, Taylor RT, Walts AD, Tsuruda S, Sakai M, Ishizuka I, Boer EF, Foster EC, Chiramel AI, Addison CB, Green R, Kastner DL, Katze MG, Holland SM, Forlino A, Freeman AF, Boehm M, Yoshii K, Best SM
  • Journal Title: Cell Host Microbe Volume: 18 Issue: 1 Pages: 61-74
  • DOI: 10.1016/j.chom.2015.06.007
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Isolation and characterization of tick-borne encephalitis virus from Ixodes persulcatus in Mongolia in 2012. (2015)
  • Author(s): Muto M, Bazartseren B, Tsevel B, Dashzevge E, Yoshii K, Kariwa H
  • Journal Title: Ticks and tick-borne diseases. Volume: 6 Issue: 5 Pages: 623-629
  • DOI: 10.1016/j.ttbdis.2015.05.006
  • NAID: 120005768779
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Virulence of tick-borne encephalitis virus is associated with intact conformational viral RNA structures in the variable region of the 3'-UTR (2015)
  • Author(s): Sakai, M., Muto, M., Hirano, M., Kariwa, H., Yoshii, K.
  • Journal Title: Virus Res Volume: 203 Pages: 36-40
  • DOI: 10.1016/j.virusres.2015.03.006
  • NAID: 120005752848
  • Peer Reviewed
• [Journal Article] Isolation of the Thogoto virus from a Haemaphysalis longicornis in Kyoto city, Japan. (2015)
  • Author(s): Yoshii K, Okamoto N, Nakao R, Hofstetter RK, Yabu T, Masumoto H, Someya A, Kariwa H, Maeda A
  • Journal Title: J Gen Virol Volume: 96 Issue: 8 Pages: 2099-2103
  • DOI: 10.1099/vir.0.000177
  • NAID: 120005820712
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evaluation of the European tick-borne encephalitis vaccine against Omsk hemorrhagic fever virus. (2014)
  • Author(s): Chidumayo NN, Yoshii K, Kariwa H
  • Journal Title: Microbiol Immunol Volume: 58 Issue: 2 Pages: 112-118
  • DOI: 10.1111/1348-0421.12122
  • NAID: 40020007920
  • Peer Reviewed
• [Journal Article] Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins. (2014)
  • Author(s): Chidumayo NN, Yoshii K, Saasa N, Sakai M, Kariwa H
  • Journal Title: Diagnostic microbiology and infectious disease Volume: 78 Issue: 4 Pages: 373-378
  • DOI: 10.1016/j.diagmicrobio.2013.12.014
  • NAID: 120005906756
  • Peer Reviewed
• [Journal Article] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures. (2014)
  • Author(s): Minato Hirano, Kentaro Yoshii, Mizuki Sakai, Rie Hasebe, Osamu Ichii and Hiroaki Kariwa
  • Journal Title: JOURNAL OF GENERAL VIROLOGY Volume: 95 Issue: 4 Pages: 849-861
  • DOI: 10.1099/vir.0.061432-0
  • Peer Reviewed
• [Journal Article] Variable region of the 3'UTR is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in a mouse model. (2014)
  • Author(s): Mizuki Sakai, Kentaro Yoshii, Yuji Sunden, Kana Yokozawa, Minato Hirano and Hiroaki Kariwa
  • Journal Title: JOURNAL OF GENERAL VIROLOGY Volume: 95 Issue: 4 Pages: 823-835
  • DOI: 10.1099/vir.0.060046-0
  • Peer Reviewed
• [Journal Article] A Critical Determinant of Neurological Disease Associated with Highly Pathogenic Tick-borne Flavivirus in Mice (2014)
  • Author(s): Yoshii K, Sunden Y, Yokozawa K, Igarashi M, Kariwa H, Holbrook MR, Takashima I.
  • Journal Title: Journal of Virology Volume: 88 Issue: 10 Pages: 5406-5420
  • DOI: 10.1128/jvi.00421-14
  • NAID: 120005496501
  • Peer Reviewed
• [Presentation] ダニ媒介性脳炎ウイルスの神経細胞内におけるウイルスゲノムRNA輸送機構の解析 (2015)
  • Author(s): 平野港、境瑞紀、苅和宏明、小林進太郎、好井健太朗
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] ウエストナイルウイルス感染による変性タンパク質蓄積機構の解析 (2015)
  • Author(s): 小林進太郎、Wallaya Phongphaew、好井健太朗、平野港、武藤芽未、大場靖子、澤洋文、苅和宏明
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] BAP31 regulates the assembly and secretory pathway of the flavivirus particles. (2015)
  • Author(s): Kentaro Yoshii，Mariko Ishizuka，Shintaro Kobayashi，Wataru Kamitani，Hiroaki Kariwa
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Survival dynamics of tick-borne encephalitis virus surrogate Langat virus in Haemaphysalis longicornis. (2015)
  • Author(s): Talactac Melbourne Rio，Kentaro Yoshii，Tetsuya Tanaka，Kozo Fujisaki，Masami Mochizuki
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Analysis of the transport mechanism of the genomic RNA of TBEV in the neurites of neuron. (2015)
  • Author(s): Minato Hirano, Mizuki Sakai, Hiroaki Kariwa, Shintaro Kobayashi, Kentaro Yoshii.
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Tick-borne flavivirus infection in main island of Japan. (2015)
  • Author(s): Hiroshi Shimoda，Kenzo Yonemitsu，Ryusei Kuwata，Daisuke Hayasaka，Kentaro Yoshii， Ken Maeda.
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Analysis of the accumulation mechanism of denatured proteins by West Nile virus infection. (2015)
  • Author(s): Shintaro Kobayashi，Phongphaew Wallaya，Kentaro Yoshii，Minato Hirano，Memi Muto， Yasuko Orba，Hirofumi Sawa，Hiroaki Kariwa.
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] ダニ媒介性脳炎ウイルスの粒子形成・分泌に関与する宿主因子の同定と機能解析 (2015)
  • Author(s): 山内沙也果, 平野港, 石塚万里子, 武藤芽未, 小林進太郎, 神谷亘, 苅和宏明, 好井健太朗
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Isolation of Puumala virus using MRK 101 cell line which derived from the kidney of the grey red-backed vole (Myodes rufocanus bedfordiae) (2015)
  • Author(s): Daichi Kanameda，Takahiro Sanada，Mizuki Sakai，Masahiro Maki，Kumiko Yoshimatsu， Jiro Arikawa，Shintaro Kobayashi，Kentaro Yoshii，Hiroaki Kariwa
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] ダニ媒介性脳炎ウイルスのウイルス様粒子を用いた動物種非特異的な新規血清学的診断法の開発 (2015)
  • Author(s): Eri Inagaki，Mizuki Sakai，Minato Hirano，Memi Muto，Shintaro Kobayashi，Hiroaki Kariwa， Kentaro Yoshii
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] ダニ媒介性脳炎ウイルスの神経細胞内におけるウイルスゲノムRNA輸送機構の解析 (2015)
  • Author(s): 平野港、境瑞紀、小林進太郎、苅和宏明、好井健太朗
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-21
• [Presentation] ダニ媒介性脳炎ウイルス様ウイルスの西日本のイノシシでの感染 (2015)
  • Author(s): 下田宙、水野純子、米満研三、南昌平、鍬田龍星、好井健太朗、早坂大輔、前田健
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-21
• [Presentation] トゴトウイルスのゲノムRNAの解析とウイルス蛋白質発現の解析 (2015)
  • Author(s): 脊戸優、佐々木創平、岡本奈津実、好井健太朗、中尾亮、染谷梓、前田秋彦
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-21
• [Presentation] ダニ媒介性脳炎ウイルスのウイルス様粒子を用いた動物種非特異的な新規血清学的診断法の開発 (2015)
  • Author(s): 稲垣恵理、境瑞紀、平野港、武藤芽未、苅和宏明、好井健太朗
  • Organizer: 第22回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2015-11-21
• [Presentation] The variable region of the 3' untranslated region is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in mouse model. (2015)
  • Author(s): Mizuki Sakai, Minato Hirano, Memi Muto, Hiroaki Kariwa, Kentaro Yoshii
  • Organizer: International Symposium on Flaviviruses: Structure and Immunity.
  • Place of Presentation: Austria, Vienna, Austria Trend Hotel Savoyen Vienna
  • Year and Date: 2015-10-08
• [Presentation] Isolation and characterization of tick-borne encephalitis virus from Ixodes persulcatus in Mongolia in 2012. (2015)
  • Author(s): Memi Muto, Boldbaatar Bazartseren, Bazartseren Tsevel, Erdenechimeg Dashzevge, Kentaro Yoshii, Hiroaki Kariwa.
  • Organizer: The 3rd Sapporo Summer Seminar for One Health
  • Place of Presentation: 北海道札幌市北海道大学獣医学部
  • Year and Date: 2015-09-16
• [Presentation] Analysis of the transport mechanism of the genomic RNA of TBEV to the neurites. (2015)
  • Author(s): Minato Hirano, Mizuki Sakai, Hiroaki Kariwa, Shintaro Kobayashi, Kentaro Yoshii
  • Organizer: The 3rd Sapporo Summer Seminar for One Health
  • Place of Presentation: 北海道札幌市北海道大学獣医学部
  • Year and Date: 2015-09-16
• [Presentation] Ecological survey of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) in Japan. (2015)
  • Author(s): Tapiwa Lundu Mtonga, Kentaro Yoshii, Hiroaki Kariwa.
  • Organizer: The 3rd Sapporo Summer Seminar for One Health
  • Place of Presentation: 北海道札幌市北海道大学獣医学部
  • Year and Date: 2015-09-16
• [Presentation] ダニ媒介性脳炎ウイルスのウイルス様粒子を用いた動物種非特異的な新規血清学的診断法の開発 (2015)
  • Author(s): 稲垣恵理、境瑞紀、平野港、武藤芽未、苅和宏明、好井健太朗
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 青森県十和田市北里大学獣医学部
  • Year and Date: 2015-09-07
• [Presentation] ムノクロマトグラフィー法によるハンタウイルスの迅速診断法の開発 (2015)
  • Author(s): 小山芽以、吉松組子、好井健太朗、有川二郎、苅和宏明
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 青森県十和田市北里大学獣医学部
  • Year and Date: 2015-09-07
• [Presentation] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus (2015)
  • Author(s): Melbourne Rio Talactac、好井健太朗、辻尚利、藤崎幸蔵、田仲哲也、望月雅美
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 青森県十和田市北里大学獣医学部
  • Year and Date: 2015-09-07
• [Presentation] Tick-borne flavivirus infection in Japan. (2015)
  • Author(s): Hiroshi Shimoda, Kenzo Yonemitsu, Ryusei Kuwata, Kentaro Yoshii, Daisuke Hayasaka, Ken Maeda
  • Organizer: The International Conference on Diseases in Nature Communicable to Man（ICDNCM）
  • Place of Presentation: USA, Montana, Rocky Mountain Laboratories
  • Year and Date: 2015-08-09
• [Presentation] ダニ媒介性脳炎ウイルスの神経細胞内におけるウイルスゲノムRNA輸送機構の解析 (2015)
  • Author(s): 平野港、境瑞紀、苅和宏明、好井健太朗
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: 北海道札幌市ホテルライフォート札幌
  • Year and Date: 2015-07-15
• [Presentation] フラビウイルス粒子形成・分泌に関与する宿主因子の検索及び機能解析 (2015)
  • Author(s): 好井健太朗、石塚万里子、神谷亘、苅和宏明.
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 京都府京都市ホテル京都エミナース
  • Year and Date: 2015-05-15
• [Presentation] 神経細胞内におけるダニ媒介性脳炎ウイルスのゲノムRNA輸送機構の解析 (2015)
  • Author(s): 平野港、境瑞紀、苅和宏明、好井健太朗
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 京都府京都市ホテル京都エミナース
  • Year and Date: 2015-05-15
• [Presentation] モンゴルにおけるダニ媒介性脳炎ウイルスの分離と性状解析 (2015)
  • Author(s): 武藤芽未、Boldbaatar Bazartseren、Bazartseren Tsevel、Erdenechimeg Dashzevge、好井 健太朗、苅和 宏明
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 京都府京都市ホテル京都エミナース
  • Year and Date: 2015-05-15
• [Presentation] 山口県のイノシシからLangatウイルスに対する抗体の検出 (2015)
  • Author(s): 下田宙、早坂大輔、好井健太郎、米満研三、鍬田龍星、高野愛、前田健
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 京都府京都市ホテル京都エミナース
  • Year and Date: 2015-05-15
• [Presentation] マダニからのThogoto virusの分離と解析 (2015)
  • Author(s): 佐々木創平、好井健太朗、岡本奈津実、中尾亮、染谷梓、前田秋彦
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 京都府京都市ホテル京都エミナース
  • Year and Date: 2015-05-15
• [Presentation] レポーター遺伝子発現ダニ媒介性脳炎ウイルスの作製と性状解析. (2014)
  • Author(s): 池端真帆, 好井健太朗, 境瑞紀, 平野港, 苅和宏明.
  • Organizer: 第62回日本ウイルス学会学術集会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-12
• [Presentation] 極東型ダニ媒介性脳炎ウイルスの高病原化に関わる3'非翻訳領域variable regionの役割. (2014)
  • Author(s): 境瑞紀, 好井健太朗, 横澤香菜, 平野港, 苅和宏明
  • Organizer: 第62回日本ウイルス学会学術集会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-12
• [Presentation] HokkaidoウイルスとPuumalaウイルスの遺伝子再集合体の作出とその性状解析. (2014)
  • Author(s): 岩崎里菜, 真田崇弘, 好井健太朗, 苅和宏明.
  • Organizer: 第62回日本ウイルス学会学術集会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-12
• [Presentation] モンゴルにおけるダニ媒介性脳炎ウイルスの分離と性状解析 (2014)
  • Author(s): 武藤芽未, Boldbaatar, B., 好井健太朗, 苅和宏明.
  • Organizer: 第62回日本ウイルス学会学術集会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-11
• [Presentation] Thogoto virus 様ウイルスのダニからの分離. (2014)
  • Author(s): 岡本奈津実, 好井健太朗, 中尾亮, Hofstetter, R. K., 藪智子, 益本大輝, 染谷梓, 前田秋彦.
  • Organizer: 第62回日本ウイルス学会学術集会.
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-11
• [Presentation] フラビウイルス粒子形成・分泌に関与する宿主因子の検索および機能解析. (2014)
  • Author(s): 好井健太朗
  • Organizer: 第21回トガ・フラビ・ペスチウイルス研究会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2014-11-09
• [Presentation] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures. (2014)
  • Author(s): Hirano, M., Yoshii, K., Sakai, M., Hasebe, R., Ichii, O., Kariwa, H.
  • Organizer: The 2nd Sapporo Summer Seminar for One Health.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-24
• [Presentation] Isolation and characterization of tick-borne encephalitis virus from Mongolia. (2014)
  • Author(s): Muto, M., Bazartseren, B., Yoshii, K., Kariwa, H.
  • Organizer: The 2nd Sapporo Summer Seminar for One Health.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-24
• [Presentation] 北海道産マダニからの新規フレボウイルスの検出 (2014)
  • Author(s): 中尾亮, 梶原将大, 邱永晋, 森亜紀奈, 直亨則, 村松美笑子, 好井健太朗, 苅和宏明, 澤洋文, 杉本千尋, 高田礼人.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-10
• [Presentation] レポーター遺伝子発現ダニ媒介性脳炎ウイルスの作製と性状解析. (2014)
  • Author(s): 池端真帆, 好井健太朗, 境瑞紀, 平野港, 苅和宏明.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-10
• [Presentation] 極東型ダニ媒介性脳炎ウイルスの高病原化に関わる3'非翻訳領域variable regionの役割. (2014)
  • Author(s): 境瑞紀, 好井健太朗, 横澤香菜, 平野港, 苅和宏明.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-09
• [Presentation] モンゴルにおけるダニ媒介性脳炎ウイルスの分離と性状解析. (2014)
  • Author(s): 武藤芽未, Boldbaatar, B., 好井健太朗, 苅和宏明.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-09
• [Presentation] 国内の野生動物およびダニから新規フラビウイルスの検出 (2014)
  • Author(s): 下田宙, 米満研三, 早坂大輔, 好井健太朗, 寺田豊, 野口慧多, 鍬田龍星, 高野愛, 前田健.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-09
• [Presentation] HokkaidoウイルスとPuumalaウイルスの遺伝子再集合体の作出とその性状解析. (2014)
  • Author(s): 岩崎里菜, 真田崇弘, 好井健太朗, 苅和宏明.
  • Organizer: 第157回日本獣医学会学術集会.
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2014-09-09
• [Presentation] Detection and isolation of novel phleboviruses from ticks in Japan. (2014)
  • Author(s): Nakao, R., Kajihara, M., Matsuno, K., Qiu, Y., Mori, A., Nao, N., Yoshii, K., Kariwa, H., Sawa, H., Sugimoto, C., Takada, A., Ebihara, H.
  • Organizer: the 12th Biennial Conference of the Society for Tropical Veterinary Medicine and the VIII International Conference on Ticks and Tick-borne Pathogens.
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-08-26
• [Presentation] Passage of Hantaan virus strain AA57 in Vero E6 cells affects pathogenicity in mice. (2014)
  • Author(s): Kariwa, H., Maki, M., Seto, T., Sanada, T., Yoshimatsu, K., Arikawa, J., Yoshii, K.
  • Organizer: International Union of Microbiological Societies 2014
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-31
• [Presentation] A critical determinant of neurological disease associated with highly pathogenic tick-borne flavivirus in mice (2014)
  • Author(s): Yoshii, K., Sunden, Y., Yokozawa, K., Igarashi, M., Kariwa, H., Holbrook, M. R., Takashima, I.
  • Organizer: International Union of Microbiological Societies 2014
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-29
• [Presentation] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures. (2014)
  • Author(s): Hirano, M., Yoshii, K., Sakai, M., Hasebe, R., Ichii, O., Kariwa, H.
  • Organizer: International Union of Microbiological Societies 2014
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-29
• [Presentation] Variable region of the 3' UTR is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in a mouse model. (2014)
  • Author(s): Sakai, M., Yoshii, K., Sunden, Y., Yokozawa, K., Hirano, M., Kariwa, H.
  • Organizer: International Union of Microbiological Societies 2014
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2014-07-29
• [Presentation] ダニ媒介性フラビウイルスのインターフェロンアンタゴニスト作用の解析 (2014)
  • Author(s): 好井健太朗, 鶴田征太郎, 境瑞紀, 苅和宏明
  • Organizer: 第49回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 山口県山口市
  • Year and Date: 2014-05-16
• [Presentation] 山口県の野生動物およびダニからフラビウイルスの検出 (2014)
  • Author(s): 下田宙, 米満研三, 早坂大輔, 好井健太朗, 寺田豊, 野口慧多, 鍬田龍星, 高野愛, 前田健.
  • Organizer: 第49回日本脳炎ウイルス生態学研究会
  • Place of Presentation: 山口県山口市
  • Year and Date: 2014-05-16