Study of pathogenic mechanism regulated by virus-derived noncoding RNA
Project/Area Number |
26660220
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
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Research Institution | Hokkaido University |
Principal Investigator |
YOSHII Kentaro 北海道大学, (連合)獣医学研究科, 准教授 (50421988)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | フラビウイルス / 脳炎 / ノンコーディングRNA / 非翻訳領域 / ダニ媒介性脳炎ウイルス |
Outline of Final Research Achievements |
Flaviviruses include many clinically important zoonotic pathogens, but little is known about the pathogenic mechanism. In our previous study, we showed that the 3’-untranslated region (UTR) is involved in the pathogenicity of the strains of tick-borne encephalitis virus (TBEV). In this study, to investigate the detailed function of the 3’-UTR, we constructed recombinant TBEV with partial deletions in the variable region of the 3’-UTR. In a mouse model, the partial deletions drastically increased the virulence of the virus, with no effect on virus multiplication in mouse brain. These data suggested that the conformational structure of the variable region is associated with the pathogenicity of TBEV. These findings provide a foundation for further research to identify the pathogenic mechanisms of TBEV.
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Report
(3 results)
Research Products
(59 results)
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[Journal Article] Flavivirus antagonism of type I interferon signaling reveals prolidase as a regulator of IFNAR1 maturation and expression.2015
Author(s)
Lubick KJ, Robertson SJ, McNally KL, Freedman BA, Rasmussen AL, Taylor RT, Walts AD, Tsuruda S, Sakai M, Ishizuka I, Boer EF, Foster EC, Chiramel AI, Addison CB, Green R, Kastner DL, Katze MG, Holland SM, Forlino A, Freeman AF, Boehm M, Yoshii K, Best SM
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Journal Title
Cell Host Microbe
Volume: 18
Issue: 1
Pages: 61-74
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Tick-borne flavivirus infection in Japan.2015
Author(s)
Hiroshi Shimoda, Kenzo Yonemitsu, Ryusei Kuwata, Kentaro Yoshii, Daisuke Hayasaka, Ken Maeda
Organizer
The International Conference on Diseases in Nature Communicable to Man(ICDNCM)
Place of Presentation
USA, Montana, Rocky Mountain Laboratories
Year and Date
2015-08-09
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[Presentation] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures.2014
Author(s)
Hirano, M., Yoshii, K., Sakai, M., Hasebe, R., Ichii, O., Kariwa, H.
Organizer
The 2nd Sapporo Summer Seminar for One Health.
Place of Presentation
北海道札幌市
Year and Date
2014-09-24
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[Presentation] 北海道産マダニからの新規フレボウイルスの検出2014
Author(s)
中尾亮, 梶原将大, 邱永晋, 森亜紀奈, 直亨則, 村松美笑子, 好井健太朗, 苅和宏明, 澤洋文, 杉本千尋, 高田礼人.
Organizer
第157回日本獣医学会学術集会.
Place of Presentation
北海道札幌市
Year and Date
2014-09-10
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[Presentation] Detection and isolation of novel phleboviruses from ticks in Japan.2014
Author(s)
Nakao, R., Kajihara, M., Matsuno, K., Qiu, Y., Mori, A., Nao, N., Yoshii, K., Kariwa, H., Sawa, H., Sugimoto, C., Takada, A., Ebihara, H.
Organizer
the 12th Biennial Conference of the Society for Tropical Veterinary Medicine and the VIII International Conference on Ticks and Tick-borne Pathogens.
Place of Presentation
Cape Town, South Africa
Year and Date
2014-08-26
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[Presentation] Passage of Hantaan virus strain AA57 in Vero E6 cells affects pathogenicity in mice.2014
Author(s)
Kariwa, H., Maki, M., Seto, T., Sanada, T., Yoshimatsu, K., Arikawa, J., Yoshii, K.
Organizer
International Union of Microbiological Societies 2014
Place of Presentation
Montreal, Canada
Year and Date
2014-07-31
Related Report
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[Presentation] A critical determinant of neurological disease associated with highly pathogenic tick-borne flavivirus in mice2014
Author(s)
Yoshii, K., Sunden, Y., Yokozawa, K., Igarashi, M., Kariwa, H., Holbrook, M. R., Takashima, I.
Organizer
International Union of Microbiological Societies 2014
Place of Presentation
Montreal, Canada
Year and Date
2014-07-29
Related Report
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[Presentation] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures.2014
Author(s)
Hirano, M., Yoshii, K., Sakai, M., Hasebe, R., Ichii, O., Kariwa, H.
Organizer
International Union of Microbiological Societies 2014
Place of Presentation
Montreal, Canada
Year and Date
2014-07-29
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[Presentation] Variable region of the 3' UTR is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in a mouse model.2014
Author(s)
Sakai, M., Yoshii, K., Sunden, Y., Yokozawa, K., Hirano, M., Kariwa, H.
Organizer
International Union of Microbiological Societies 2014
Place of Presentation
Montreal, Canada
Year and Date
2014-07-29
Related Report
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