Host factors affecting the susceptibility of neurons to flavivirus: an approach from cellular differentiation

• Project/Area Number: 26660222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA TAKASHI 北海道大学, (連合)獣医学研究科, 教授 (90261338)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 日本脳炎ウイルス / 神経細胞 / ウイルス感受性

Outline of Final Research Achievements

CSM14.1 is a rat mesencephalic neuronal cell line that can be induced to differentiate into neurons by culture under non-permissive conditions. CSM14.1 cells become more resistant to Japanese encephalitis virus (JEV) infection as they mature. Differentiation-dependent gene expression in CSM14.1 cells was analyzed by microarray. To identify host molecules that is responsible for the differentiation-dependent susceptibility of neuronal cells with JEV infection, we cloned two genes whose expression in undifferentiated CSM14.1 cell was higher than that in differentiated CSM14.1 cells, and also cloned other two genes whose expression in differentiated CSM14.1 cell was higher than that in undifferentiated CSM14.1 cells. Effect of expression of those genes in undifferentiated CSM14.1 cells on JEV-susceptibility was analyzed.

Research Products

• [Journal Article] Detection of novel polyomaviruses in fruit bats in Indonesia. (2015)
  • Author(s): Kobayashi S. et al.
  • Journal Title: Archives of Virology Volume: 160 Issue: 4 Pages: 1075-1082
  • DOI: 10.1007/s00705-015-2349-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Detection of coronavirus genomes in Moluccan naked-backed fruit bats in Indonesia. (2015)
  • Author(s): Anindita P. D. et al.
  • Journal Title: Archives of Virology Volume: 160 Issue: 4 Pages: 1113-1118
  • DOI: 10.1007/s00705-015-2342-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Autophagy inhibits viral genome replication and gene expression stages in West Nile virus infection. (2014)
  • Author(s): Kobayashi S, Orba Y, Yamaguchi H, Takahashi K, Sasaki M, Hasebe R, Kimura T, Sawa H.
  • Journal Title: Virus Res Volume: 191 Pages: 83-91
  • DOI: 10.1016/j.virusres.2014.07.016
  • NAID: 120005517727
  • Peer Reviewed
• [Journal Article] Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport (2013)
  • Author(s): Makino Y, Suzuki T, Hasebe R, Kimura T, Maeda A, Takahashi H, Sawa H
  • Journal Title: J Virol Methods Volume: 195 Pages: 250-257
  • DOI: 10.1016/j.jviromet.2013.10.002
  • Peer Reviewed