| Project/Area Number |
26660227
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ウイルス / CRISPR/Cas9 / ゲノム編集 |
|---|
| Outline of Final Research Achievements |
Cellular factors are essential for viral growth. To identify these factors, we used a novel genome editing CRISPR/Cas9 system in combination of viral vectors. We constructed a retroviral vector library expressing random targeting gRNA/Cas9 and infected cell culture with the library. Then, following infection of influenza or Akabane virus, we selected cells resistant to virus infections. Through these procedures, we identified heparan sulfate proteoglycan as an cellular attachment factor for Akabane virus.
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