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Identification of cellular factors responsible for virus growth by a novel genome editing technology

Research Project

Project/Area Number 26660227
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

Horimoto Taisuke  東京大学, 農学生命科学研究科, 教授 (00222282)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsウイルス / CRISPR/Cas9 / ゲノム編集
Outline of Final Research Achievements

Cellular factors are essential for viral growth. To identify these factors, we used a novel genome editing CRISPR/Cas9 system in combination of viral vectors. We constructed a retroviral vector library expressing random targeting gRNA/Cas9 and infected cell culture with the library. Then, following infection of influenza or Akabane virus, we selected cells resistant to virus infections. Through these procedures, we identified heparan sulfate proteoglycan as an cellular attachment factor for Akabane virus.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Molecular epidemiological analyses of Cryptosporidium parvum virus 1 (CSpV1), asymbiotic virus of Cryptosporidium parvum, in Japan2016

    • Author(s)
      Murakoshi F, Ichikawa-Seki M, Aita J, Yaita S, Kinami A, Fujimoto K, Nishikawa Y, Murakami S, Horimoto T, Kato K.
    • Journal Title

      Virus Res.

      Volume: 211 Pages: 69-72

    • DOI

      10.1016/j.virusres.2015.09.021

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crimean-Congo hemorrhagic fever virus entry mechanism, using a vesicular stomatitis virus pseudotyping system.2016

    • Author(s)
      Suda Y, Horimoto T, et al.
    • Journal Title

      Arch. Virol.

      Volume: 211

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physical interaction between bovine viral diarrhea virus nonstructural protein 4A and adenosine deaminase acting on RNA (ADAR).2014

    • Author(s)
      Mohamed YM, Horimoto T, et al.
    • Journal Title

      Arch. Virol.

      Volume: 159 Issue: 7 Pages: 1735-1741

    • DOI

      10.1007/s00705-014-1997-3

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 牛ウイルス性下痢ウイルス非構造蛋白質のオートファゴソーム形成誘導の解析2015

    • Author(s)
      須田遊人、堀本泰介ら
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] アカバネウイルス感染におけるヘパラン硫酸プロオグリカンの役割2015

    • Author(s)
      村上晋、堀本泰介ら
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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