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The preparation of gene knock out tick by TALEN using nuclease for infectious model

Research Project

Project/Area Number 26660229
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionKagoshima University

Principal Investigator

TANAKA TETSUYA  鹿児島大学, 農水産獣医学域獣医学系, 准教授 (00322842)

Co-Investigator(Renkei-kenkyūsha) MOCHIZUKI MASAMI  鹿児島大学, 農水産獣医学域獣医学系, 教授 (90157834)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsマダニ / マダニ細胞 / アクチンプロモータ- / フェリチンプロモーター / CAGプロモーター / PGKプロモーター / ゲノム / プロモーター領域 / アクチン / チュブリン / ディフェンシン / PGK / フェリチン / 昆虫細胞
Outline of Final Research Achievements

Although tick studies were conducted by gene silencing using RNA interferance for analyzing the gene function of tick, the skills of gene knockeout and gene transgenic for ticks have not been developing at the morment.Therefore, we tried to incorporate foreign gene into the tick cell line for establishing gene enginering. Although the effect of gene plasmid transfeciton into the tick cell line showed high luceferase activity using pmiGLO or pCAGGS-Luceferase,the effect of plasmid transfection into the tick cell line showed low luceferase activity using actin or ferritin pomotor.These studies indicated that the foreign gene incorporated into the tick may make a break through the praparation of gene konockout or trangenic tick.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (37 results)

All 2016 2015 2014 Other

All Journal Article (13 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 13 results,  Open Access: 3 results,  Acknowledgement Compliant: 8 results) Presentation (18 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (4 results) Remarks (2 results)

  • [Journal Article] Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice2016

    • Author(s)
      Yuina Ono, Makoto Matsubayashi, Hiroaki Kawaguchi, Masashi Tsujio, Masanobu Mizuno, Tetsuya Tanaka, Tatsunori Masatani, Toshihiro Matsui, Tomohide Matsuo
    • Journal Title

      Acta Parasitologica

      Volume: 115(1) Issue: 1 Pages: 211-5

    • DOI

      10.1007/s00436-015-4737-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection.2016

    • Author(s)
      Takechi R, Galay RL, Matsuo T, Maeda H, Kusakisako K, Talactac MR, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Ticks and Tick-borne diseases

      Volume: 7 Issue: 1 Pages: 36-45

    • DOI

      10.1016/j.ttbdis.2015.08.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria.2016

    • Author(s)
      Maeda H, Miyata T, Kusakisako K, Galay RL, Talactac MR, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 57 Pages: 38-47

    • DOI

      10.1016/j.dci.2015.12.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Impaired cellular immune response to injected bacteria after knockdown of ferritin genes in the hard tick Haemaphysalis longicornis.2016

    • Author(s)
      Galay RL, Takechi R, Umemiya-Shirafuji R, Talactac MR, Maeda H, Kusakisako K, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Parasitology International

      Volume: 65 Issue: 3 Pages: 251-257

    • DOI

      10.1016/j.parint.2016.01.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus.2016

    • Author(s)
      Talactac MR, Yoshii K, Maeda H, Kusakisako K, Hernandez EP, Tsuji N, Fujisaki K, Galay RL, Tanaka T, Mochizuki M.
    • Journal Title

      Parasit Vectors

      Volume: 9 Issue: 1 Pages: 59-59

    • DOI

      10.1186/s13071-016-1344-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media2016

    • Author(s)
      Kusakisako, K., Masatani, T., Yada Y., Talactac, M. R., Hernandez, E. P., Maeda, H., Mochizuki, M. and Tanaka, T.
    • Journal Title

      Parasitol. Int.

      Volume: In press Issue: 5 Pages: 532-535

    • DOI

      10.1016/j.parint.2016.02.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA2016

    • Author(s)
      Galay, R. L., Hernandez, E. P., Talactac, M. R., Maeda, H., Kusakisako, K., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Ticks Tick Borne Dis.

      Volume: In press Issue: 5 Pages: 813-816

    • DOI

      10.1016/j.ttbdis.2016.03.018

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis2015

    • Author(s)
      Kusakisako K, Masatani T, Miyata T, Galay RL, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Insect Molecular Biology

      Volume: 25 Issue: 1 Pages: 16-23

    • DOI

      10.1111/imb.12193

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis.2015

    • Author(s)
      Maeda H, Kurisu K, Miyata T, Kusakisako K, Galay RL, Rio TM, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Parasitol. Res.

      Volume: 114 Issue: 5 Pages: 1793-1802

    • DOI

      10.1007/s00436-015-4365-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The widely distributed hard tick, <i>Haemaphysalis longicornis</i>, can retain canine parvovirus, but not be infected in laboratory condition2015

    • Author(s)
      Mori H., Tanaka, T. and Mochizuki, M.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 77 Issue: 4 Pages: 405-411

    • DOI

      10.1292/jvms.14-0199

    • NAID

      130005067022

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The vector potential of Haemaphysalis longicornis ticks for Babesia microti parasites under experimental condition2015

    • Author(s)
      Kusakisako, K., Maeda, H., Galay, R. L., Matsuo, T., Tsujio, M., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Journal Title

      J. Protozool. Res.

      Volume: In press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis2014

    • Author(s)
      Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Maeda, H., Kusakisako, K., Tsuji, N., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Parasit. Vectors

      Volume: 7(1) Pages: 482-482

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet2014

    • Author(s)
      Galay, R. L., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Journal Title

      Parasitol. Int.

      Volume: 64(2) Issue: 2 Pages: 182-189

    • DOI

      10.1016/j.parint.2014.12.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 病原微生物感染に関与するマダニ生物活性分子について2015

    • Author(s)
      田仲哲也
    • Organizer
      日本衛生動物学会・日本ダニ学会共催 公開シンポジウム
    • Place of Presentation
      法政大学(千代田区、東京都)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus2015

    • Author(s)
      Melbourne Rio Talactac, 好井健太朗, 辻 尚利, 藤崎幸蔵, 田仲哲也, 望月雅美
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学(八戸市、青森)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Knockdown of ferritin genes increases the susceptibility of the hard tick Haemaphysalis longicornis to bacterial infection2015

    • Author(s)
      Tetsuya Tanaka, Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji, Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki
    • Organizer
      World Association for the Advancement of Veterinary Parasitology Congress 2015
    • Place of Presentation
      The Arena and Convention Centre(Liverpool, UK)
    • Year and Date
      2015-08-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Decreased resistance of Haemaphysalis longicornis ticks to bacterialchallenge after knockdown of ferritin molecules2015

    • Author(s)
      Remil Linggatong Galay, 武智理恵, 白藤(梅宮)梨可, Melbourne Rio Talactac, 前田大輝, 草木迫浩大, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第23回SADI
    • Place of Presentation
      名取市文化会館(名取市、宮城)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] フタトゲチマダニ由来ロイシンリッチリピート保有タンパク質(HlLRR)の発現動態とバベシア原虫に及ぼす影響2015

    • Author(s)
      田仲哲也, 栗巣孔士, 前田大輝, 宮田 健, 草木迫浩大, Remil Linggatong Galay, Melbourne Rio Talactac, 望月雅美, 藤崎幸蔵
    • Organizer
      第23回SADI
    • Place of Presentation
      名取市文化会館(名取市、宮城)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] フタトゲチマダニ由来2-Cys型ペルオキシレドキシン (HlPrx2) の抗酸化部位の検索2015

    • Author(s)
      草木迫浩大, 正谷達謄, 宮田 健, Remil Linggatong Galay, 前田大輝, 辻 尚利, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学(三鷹市、東京都)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] The role of ferritin in immunity of the ixodid tick Haemaphysalis longicornis injected with Escherichia coli2015

    • Author(s)
      Remil Linggatong Galay, Rie Takechi, Rika Umemiya-Shirafuji,Melbourne Rio Talactac, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学(三鷹市、東京都)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of the antioxidant active sites of Haemaphysalis longicornis 2-Cys type peroxiredoxin (HlPrx2)2015

    • Author(s)
      Kodai Kusakisako, Tatsunori Masatani, Takeshi Miyata, Remil Linggatong Galay, Hiroki Maeda, Naotoshi Tsuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      Joint Symposium of the 6th Veterinary Research and the 3rd Veterinary Education among Universities of Veterinary Medicine in East Asia
    • Place of Presentation
      台湾大学(台北、台湾)
    • Year and Date
      2015-02-02 – 2015-02-03
    • Related Report
      2014 Research-status Report
  • [Presentation] ラクトフェリンによる抗マラリア原虫機能の解析2014

    • Author(s)
      小森園 亮, 柿本麻美子, 田仲哲也, 李 長春, 渡部久実, Ibrahim Hisham, 若林裕之,山内恒治, 宮田 健
    • Organizer
      第19回日本フードファクター学会
    • Place of Presentation
      鹿児島大学(鹿児島市、鹿児島県)
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification and characterization of a C-type lectin like protein from the hard tick Haemaphysalis longicornis2014

    • Author(s)
      Hiroki Maeda, Takeshi Miyata, Kodai Kusakisako, Remil Linggatong Galay, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      The 2nd Sapporo Summer Seminar for One Health
    • Place of Presentation
      北海道大学(札幌市、北海道)
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control2014

    • Author(s)
      Galay Remil Linggatong, 白藤(梅宮)梨可, 宮田 健, 草木迫浩大, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市、北海道)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] フタトゲチマダニ由来2-Cys型ペルオキシレドキシンの機能解析2014

    • Author(s)
      草木迫浩大, 正谷達謄, Galay Remil Linggatong, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市、北海道)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] フタトゲチマダニ由来TNF Receptor Associated Factor(TRAF)の同定とその役割について2014

    • Author(s)
      武智理恵, Galay Remil Linggatong, 前田大輝, 草木迫浩大, 松尾智英, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市、北海道)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] フタトゲチマダニ由来C型レクチン様蛋白質の動態および機能解析2014

    • Author(s)
      前田大輝, 宮田健, 草木迫浩大, Remil Linggatong Galay, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第22回分子寄生虫学ワークショップ第12回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学(帯広市、北海道)
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2014 Research-status Report
  • [Presentation] フタトゲチマダニ由来 2-Cys 型ペルオキシレドキシンの動態2014

    • Author(s)
      草木迫浩大, 正谷達謄, Remil Linggatong Galay, 前田大輝, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第22回分子寄生虫学ワークショップ第12回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学(帯広市、北海道)
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2014 Research-status Report
  • [Presentation] The potential of recombinant ferritin 1 and ferritin 2 as anti-tick vaccine against Haemaphysalis longicornis2014

    • Author(s)
      Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      TTP8: Ticks and Tick-Borne Pathogens
    • Place of Presentation
      Cape Sun hotel(Cape Town, South Africa)
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] The iron-storage and antioxidant functions of two types of ferritin are critical to successful blood feeding and reproduction of the ixodid tick Haemaphysalis longicornis2014

    • Author(s)
      Galay Remil Linggatong, Umemiya-Shirafuji Rika, Maeda Hiroki, Kusakisako Kodai, Mochizuki Masami, Fujisaki Kozo, Tanaka Tetsuya
    • Organizer
      The XIIIth International Congress of Parasitoplogy
    • Place of Presentation
      Camino Real Hotel(Mexico City,Mexico)
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Research-status Report
  • [Presentation] Ticks survive at a threshold between engorgement and starvation2014

    • Author(s)
      Kozo Fujisaki, Naotoshi Tsuji, Xuenan Xuan, Rika Shirafuji-Umemiya, Damdinsuren Boldbaatar, Tetsuya Tanaka, Takeshi Hatta, Banzragch Battur, Badgar Battsetseg
    • Organizer
      14th International Congress of Acarology
    • Place of Presentation
      京都テルサ(京都市、京都府)
    • Year and Date
      2014-07-14 – 2014-07-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] Vaccine Design: Methods and Protocols2016

    • Author(s)
      Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Total Pages
      848
    • Publisher
      Springer New York
    • Related Report
      2015 Annual Research Report
  • [Book] RNA Interference2016

    • Author(s)
      Galay, R. L., Miyata, T., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K. and Tanaka, T.
    • Total Pages
      460
    • Publisher
      InTech-Open Access Publisher
    • Related Report
      2015 Annual Research Report
  • [Book] 第2章 免疫の概念(獣医学教育モデル・コア・カリキュラム準拠 獣医免疫学)2015

    • Author(s)
      田仲哲也、池田輝雄、岡本まり子、松本安喜(編集:池田輝雄、小川健司、松本安喜)
    • Total Pages
      17
    • Publisher
      (株)緑書房
    • Related Report
      2014 Research-status Report
  • [Book] 第6章 節足動物各論I(ダニ)(獣医学教育モデル・コア・カリキュラム準拠 寄生虫病学)2014

    • Author(s)
      田仲哲也 (編集:堀井洋一郎、野中成晃、加藤大智、平 恵介、松本 淳、横山直明)
    • Total Pages
      5
    • Publisher
      (株)緑書房
    • Related Report
      2014 Research-status Report
  • [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/research.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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