Development of a novel therapeutic strategy for canine inflammatory bowel disease using selective lymphocyte induction

• Project/Area Number: 26660237
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: Koshino Yuko (GOTO Yuko) 東京大学, 農学生命科学研究科, 特任准教授 (80436518)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ラパマイシン / 犬 / Treg / リンパ球 / 炎症性腸疾患 / Th17

Outline of Final Research Achievements

The aim of this study was; 1. To evaluate the effect of Rapamycin in canine Inflammatory bowel disease as a novel therapeutic drug; 2. To investigate the involvement of Treg and Th17 cells in clinical pahology of canine inflammatory bowel disease.
We added Rapamycin, IL-2 and TGFb to ConA-stimulated canine peripheral lymphocytes in various combination. However, compared to lymphocytes cultured with ConA alone, neither of the combination achieved significant increase in Treg cell percentage nor numbers. It might be necessary to evaluate culture condition other than ConA stimulation-based method.

Research Products

• [Journal Article] Detection of circulating tumor cells using GeneScan analysis for antigen receptor gene rearrangements in canine lymphoma patients (2016)
  • Author(s): Hiyoshi-Kanemoto, S., Goto-Koshino, Y., Fukushima, K., Takahashi, M., Kanemoto, H., Uchida, K., Fujino, Y., Ohno, K. and Tsujimoto, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 5 Pages: 877-881
  • DOI: 10.1292/jvms.15-0433
  • NAID: 130005154308
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mutation of p53 gene and its correlation with the clinical outcome in dogs with lymphoma. (2016)
  • Author(s): Koshino, A., Goto-Koshino, Y., Setoguchi, A., Ohno, K. and Tsujimoto, H.
  • Journal Title: J. Vet. Intern. Med. Volume: 30 Issue: 1 Pages: 223-229
  • DOI: 10.1111/jvim.13807
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Prognostic factors in dogs with protein-losing enteropathy. (2015)
  • Author(s): Nakashima K, Hiyoshi S, Ohno K, Uchida K, Goto-Koshino Y, Maeda S, Mizutani N, Takeuchi A, Tsujimoto H.
  • Journal Title: Vet J Volume: 205(1) Issue: 1 Pages: 28-32
  • DOI: 10.1016/j.tvjl.2015.05.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression profile of circulating serum microRNAs in dogs with lymphoma. (2015)
  • Author(s): Fujiwara-Igarashi A, Igarashi H, Mizutani N, Goto-Koshino Y, Takahashi M, Ohno K, Tsujimoto H.
  • Journal Title: Vet J Volume: 205 Issue: 2 Pages: 317-321
  • DOI: 10.1016/j.tvjl.2015.04.029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association between lymphocyte antigen receptor gene rearrangements and histopathological evaluation in canine chronic enteropathy. (2015)
  • Author(s): Hiyoshi S, Ohno K, Uchida K, Goto-Koshino Y, Nakashima K, Fukushima K, Kanemoto H, Maeda S, Tsujimoto H.
  • Journal Title: Vet Immunol Immunopathol. Volume: 165(3-4) Issue: 3-4 Pages: 138-144
  • DOI: 10.1016/j.vetimm.2015.03.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Construction of a multicolor GeneScan analytical system to detect clonal rearrangements of immunoglobulin and T cell receptor genes in canine lymphoid tumors. (2015)
  • Author(s): Goto-Koshino, Y., Mochizuki, H., Sato, M., Nakashima, K., Hiyoshi, S., Fujiwara-Igarashi, A., Maeda, S., Nakamura, K., Uchida, K., Fujino, Y., Ohno, K. and Tsujimoto, H.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: In press Issue: 1-2 Pages: 81-87
  • DOI: 10.1016/j.vetimm.2015.03.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of the drug sensitivity and expression of 16 drug resistance-related genes in canine histiocytic sarcoma cell lines (2015)
  • Author(s): Asada, H., Tomiyasu, H., Goto-Koshino, Y., Fujino, Y., Ohno, K. and Tsujimoto, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 6 Pages: 677-684
  • DOI: 10.1292/jvms.14-0415
  • NAID: 130005085856
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional analysis of pattern recognition receptors in miniature dachshunds with inflammatory colorectal polyps (2015)
  • Author(s): Igarashi H, Ohno K, Fujiwara-Igarashi A, Kanemoto H, Fukushima K, Goto-Koshino Y, Uchida K, Tsujimoto H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 4 Pages: 439-447
  • DOI: 10.1292/jvms.14-0505
  • NAID: 130005067028
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intestinal Protease-Activated Receptor-2 and Fecal Serine Protease Activity are Increased in Canine Inflammatory Bowel Disease and May Contribute to Intestinal Cytokine Expression (2014)
  • Author(s): Maeda S, Ohno K, Uchida K, Igarashi H, Goto-Koshino Y, Fujino Y, Tsujimoto H
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 8 Pages: 1119-1127
  • DOI: 10.1292/jvms.14-0060
  • NAID: 130004432195
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Differential expression of CD45 isoforms in canine leukocytes (2014)
  • Author(s): Goto-Koshino Y, Tomiyasu H, Suzuki H, Tamamoto T, Mizutani N, FujinoY, Ohno K, Tsujimoto H.
  • Journal Title: Vet Immunol Immunopathol Volume: 160 Issue: 1-2 Pages: 118-122
  • DOI: 10.1016/j.vetimm.2014.03.011
  • Peer Reviewed
• [Journal Article] Plasma Transferrin Concentration as a Nutritional Marker in Malnourished Dogs with Nutritional Treatment (2014)
  • Author(s): Nakajima M, Ohno K, Goto-Koshino Y, Fujino Y, Tsujimoto H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 4 Pages: 539-543
  • DOI: 10.1292/jvms.13-0583
  • NAID: 130003382416
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access