Development of a new diagnostic procedure for bovine leukemia virus infection using bovine milk exosomes
| Project/Area Number |
26660239
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Gifu University |
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Principal Investigator |
Inoshima Yasuo 岐阜大学, 応用生物科学部, 准教授 (20355184)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エキソソーム / 生乳 / バルク乳 / 牛白血病 / 牛白血病ウイルス / 診断法 |
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| Outline of Final Research Achievements |
We tried to establish a method for efficient isolation and purification of exosomes from bovine raw milk and to develop a new diagnostic procedure for bovine leukemia virus (BLV) infection using milk exosomes. Our study revealed that commercially available exosome-isolation kits are applicable to isolate bovine milk exosomes after removal of milk casein by sequential ultracentrifugation. BLV antigens and DNA were not detected from milk exosomes from BLV-infected young cattle subclinically. These results suggest that detectability of BLV antigens and DNA in milk exosomes are conceivably related to the copy numbers in cattle.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Identification and characterization of a multispecific monoclonal antibody G2 against chicken prion protein2014
Author(s)
Kamatari, Y. O., Ohta, S., Inoshima, Y., Oda, M., Maruno, T., Kobayashi, Y., Ishiguro, N.
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Journal Title
Protein Science
Volume: 23
Issue: 8
Pages: 1050-1059
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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