| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
Detection and counting of the circulating tumor cells (CTC) could be one of the potential approaches to predict early metastasis of cancer cells. In the present study, we therefore attempted to establish FACS-based CTC counting system using canine peripheral bold spiked with various number of model cancer cells (MDCK cells); however, the counting accuracy was not sufficient for evaluation of the number of CTC due to white bold contaminations. We then examined quantification of CTC using quantitative PCR method. Among epithelial cell-associated 35 molecules examined, CK19, AGR2, IRX3, UPKB1, and TACC2 were detected by this method only in blood spiked with MDCK cells. Particularly, CK19 and TACC2 were detected at the minimum concentration of 1 MDCK cell/1 ml blood. From these findings, CK19 and TACC2 could be potential markers for detecting CTC by quantitative PCR method.
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