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Identification and analysis for the function of maternal factors using CRISPR/Cas system

Research Project

Project/Area Number 26660246
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionThe University of Tokyo

Principal Investigator

AOKI Fugaku  東京大学, 大学院新領域創成科学研究科, 教授 (20175160)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords母性因子 / 初期発生 / 初期胚 / CRISPR/Cas9 / RNAシーケンス / ノックアウト / 卵 / CRISPR/Cas
Outline of Final Research Achievements

The purpose of this study was to develop the experimental system using RNA sequence and CRISPR/Cas9 to identify the maternal factors involved in the regulation of preimplantation development. First, we selected the genes which are specifically expressed in oocytes and whose encoding mRNAs are rapidly degraded after fertilization. Second, we chose 4 genes from those genes and made a deletion in them by CRISPR/Cas9. In one of these 4 genes, a deletion caused unusually tight chromatin structure in the oocytes and 1-cell stage embryos. Thus, we have developed the useful system to identify the maternal factors.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results) (of which Invited: 1 results)

  • [Presentation] 母性mRNAの分解に着目した減数分裂調節因子の同定とその機能解析2014

    • Author(s)
      青木不学、鈴木元
    • Organizer
      生殖細胞のエピゲノムダイナミクスとその制御・第2回領域シンポジウム
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2014-10-31 – 2014-11-01
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2017-05-10  

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