Development of a novel proteinic adjuvant

Research Project

Project/Area Number	26660247
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Integrative animal science
Research Institution	The University of Tokyo
Principal Investigator	Goto Yasuyuki 東京大学, 農学生命科学研究科, 准教授 (50553434)
Project Period (FY)	2014-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	ワクチン / アジュバント / MRP14
Outline of Final Research Achievements	Myeloid-related protein 14 (MRP14) is a protein expressed by immune cells including neutrophils and macrophages. In this study, we produced a recombinant MRP14 using an E. coli expression system and examined for its immunostimulatory activities and potency as an adjuvant for a flu vaccine. MRP14 displayed stimulatory activity on MΦ in vitro. It was revealed that MRP14 can serve as an agonist for multiple TLRs. Mice inoculated with a flu vaccine + MRP14 showed higher antibody titers than those with the vaccine alone. These suggest that MRP14 is a promising proteinic adjuvant.

Report

(3 results)