| Project/Area Number |
26660253
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Matsubayashi Makoto 大阪府立大学, 生命環境科学研究科(系), 准教授 (00321076)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 獣医学 / 原虫感染症 / シゾント |
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| Outline of Final Research Achievements |
Chicken coccidiosis is caused by Eimeria spp. is characterized by watery or hemorrhagic diarrhea, resulting in death in severe cases. E. tenella is recognized as the most significant pathogen in the genus. We compared the differential transcription in the Eimerian parasitic stage sporozoite of virulent and precocious strains to gain biological insight into the pathogenicity and precocity. Approximately 90% of the reads from both strains were mapped to the genome, and 16,630 estimated transcript regions were identified. Genes related to proteases, cell attachment proteins, mitochondrial proteins, and transporters were most strongly upregulated in the virulent strain. The expression of genes associated with cell survival, development, or proliferation was strongly upregulated in the precocious strain. These findings suggest that virulent strains survive long before invasion and invade actively/successfully into host cells, whereas proliferative processes appear to affect precocity.
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