Studies on mechanism of schizont formation of gut parasitic coccidian protozoa
Project/Area Number |
26660253
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Integrative animal science
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Research Institution | Kitasato University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
Matsubayashi Makoto 大阪府立大学, 生命環境科学研究科(系), 准教授 (00321076)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 獣医学 / 原虫感染症 / シゾント |
Outline of Final Research Achievements |
Chicken coccidiosis is caused by Eimeria spp. is characterized by watery or hemorrhagic diarrhea, resulting in death in severe cases. E. tenella is recognized as the most significant pathogen in the genus. We compared the differential transcription in the Eimerian parasitic stage sporozoite of virulent and precocious strains to gain biological insight into the pathogenicity and precocity. Approximately 90% of the reads from both strains were mapped to the genome, and 16,630 estimated transcript regions were identified. Genes related to proteases, cell attachment proteins, mitochondrial proteins, and transporters were most strongly upregulated in the virulent strain. The expression of genes associated with cell survival, development, or proliferation was strongly upregulated in the precocious strain. These findings suggest that virulent strains survive long before invasion and invade actively/successfully into host cells, whereas proliferative processes appear to affect precocity.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Transcriptional profiles of virulent and precocious strains of Eimeria tenella at sporozoite stage; novel biological insight into attenuated asexual development2016
Author(s)
Makoto Matsubayashi, Fumiya Kawahara, Takeshi Hatta, Junya Yamagishi, Takeharu Miyoshi, Anisuzzaman, Kazumi Sasai, Takashi Isobe, Kiyoshi Kita, Naotoshi Tsuji
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Journal Title
Infection, Genetics and Evolution
Volume: 40
Pages: 54-62
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DB-AT: a 2015 update to the Full-parasites database brings a multitude of new transcriptomic data for apicomplexan parasites2014
Author(s)
Jakalski, M, Wakaguri H, Kischka T, Nishikawa Y, Kawazu S, Matsubayashi M, Kawahara F, Tsuji N, Cao S, Sunaga F, Xuan X, Okubo K, Igarashi I, Tuda J, Mongan A, Eshita Y, Maeda R, Makalowski W, Suzuki Y, Yamagishi J
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Journal Title
Nucleic Acids Research
Volume: 43
Issue: D1
Pages: D631-D636
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Eimeria tenella スポロゾイトのミトコンドリア精製法の確立と呼吸鎖酵素活性2014
Author(s)
稲岡ダニエル健, 松林誠,小松谷啓介, 八田岳士, 三好猛晴, 磯部尚, 川原史也, 山岸潤也, 彦坂健児, 佐藤暖, 志波智生, 原田繁春, 辻尚利, 北潔
Organizer
第87回日本生化学会大会
Place of Presentation
国立京都国際会館・グランドプリンスホテル(京都府・京都市)
Year and Date
2014-10-16
Related Report
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[Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella2014
Author(s)
Matsubayashi M, Kawahara F, Hatta T, Miyoshi T, Yamagishi J, Anisuzzaman, Shimura K, Isobe T, Kita K, Iwata A, Tsuji N
Organizer
18th Japanese-German Cooperative Symposium on Protozoan Diseases
Place of Presentation
Doresden(Germany)
Year and Date
2014-10-01
Related Report
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[Presentation] A novel biological insight into the comparative transcriptional profiling between virulent and precocious strains at invasive stage of Eimeria tenella2014
Author(s)
Matsubayashi M, Kawahara F, Hatta T, Miyoshi T, Yamagishi J, Anisuzzaman, Shimura K, Isobe T, Kita K, Iwata A, Tsuji N
Organizer
11th International Coccidiosis Conference
Place of Presentation
Doresden(Germany)
Year and Date
2014-09-28
Related Report
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[Presentation] Eimeria tenellaスポロゾイト呼吸鎖の生化学的解析2014
Author(s)
稲岡ダニエル健, 松林誠, 小松谷啓介, 八田岳士, 三好猛晴, 磯部尚, 川原史也, 山岸潤也, 彦坂健児, 佐藤暖, 志波智生, 原田繁春, 辻尚利, 北潔
Organizer
第12回分子寄生虫マラリアフォーラム
Place of Presentation
帯広畜産大学(北海道・帯広市)
Year and Date
2014-09-01
Related Report