| Project/Area Number |
26660256
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金子 武人 京都大学, 医学研究科, 特定講師 (30332878)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 産み分け / CRISPR/Cas9 / ゲノム編集 / ノックイン / Crispr/Cas9 / 性染色体 / 受精阻害 |
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| Outline of Final Research Achievements |
In this study, using the Crispr / Cas 9 system, which is a genome editing technique developed in recent years, we knock-in genes that inhibit fertilization on sex chromosomes and inhibit fertilization of sperm having this gene, and aimed to develop technologies that enable birth separation. For this time, we planned the creation of male individuals having the desired gene in the X chromosome and not being able to fertilize sperm with X chromosome, that is, producing only male. As a result, genes were inserted in 3 lineage males, but male individuals bearing only males could not be obtained. Mating experiments are continuing also in other male lines, and in the recombinant female lines, a lineage of male recombinant mice is going to be established by crossing with wild type males.
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