| Project/Area Number |
26660258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
Ono Etsuro 九州大学, 医学(系)研究科(研究院), 教授 (00160903)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA MASATOSHI 静岡県畜産技術研究所, 中小家畜研究センター, 研究統括監 (70501443)
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| Co-Investigator(Renkei-kenkyūsha) |
URADE YOSHIHIRO 筑波大学, 医学医療系, 教授 (10201360)
KAWARASAKI TATSUO 東海大学, 農学部, 教授 (70500247)
IWAMORI NAOKI 九州大学, 大学院医学研究院, 助教 (70647362)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ゲノム編集 / 筋ジストロフィー / 疾患モデル / マイクロミニブタ |
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| Outline of Final Research Achievements |
Duchenne muscular dystrophy (DMD) is an X-linked lethal muscle disorder caused by mutations in the Dmd gene encoding Dystrophin. DMD model animals, such as mdx mice and canine X-linked muscular dystrophy dogs, have been widely utilized in the development of a treatment for DMD. Here, we have tried to generate Dmd-mutated micro-miniature pigs using CRISPR/Cas9 systems. To introduce mutations in the micro-miniature pig Dmd gene, located in the X chromosome, we predicted the sequence of exons based on information of the pig Dmd gene, designed two sgRNAs targeting exon 23 of the pig Dmd gene, and simultaneously injected the plasmids, sgRNAs and/or Cas9 proteins into zygotes. Total 7 trials were performed to generate Dmd-mutated micro-miniature pigs using CRISPR/Cas9 systems. In the 6th trial, three piglets were born, but one of them died after their birth due to a severe cardiac deformity. Unfortunately, no mutation was observed in the piglets.
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