| Project/Area Number |
26660261
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kindai University |
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Principal Investigator |
KATO Yoko 近畿大学, 農学部, 教授 (40278742)
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| Co-Investigator(Renkei-kenkyūsha) |
TANI Tetsuya 近畿大学, 農学部, 講師 (70319763)
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| Research Collaborator |
TAGUCHI Misato 近畿大学, 大学院・農学研究科
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 凍結乾燥 / 初期胚 / 哺乳動物 / 凍結保存 / 保存 / 胚盤胞 / マウス / 凍結 / 発生工学 / 受精卵 |
|---|
| Outline of Final Research Achievements |
In this study, we examined whether pre-implantation stage embryos in mammals could be freeze-dried like sperm and somatic cells. When mouse blastocysts were freeze-dried, trehalose supplemented medium for freeze-drying and isotonic solution for rehydration were effective for their blastocoele recovery. For porcine blastocysts, no blastocysts recovered their blastocoele after rehydration in any approach.
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