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Development of long term preservation for silkworm resources using CAS theory

Research Project

Project/Area Number 26660270
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionKyushu University

Principal Investigator

Banno Yutaka  九州大学, 農学研究院, 教授 (50192711)

Research Collaborator FUKUMORI Hirohisa  九州大学, 大学院農学研究院, 学術研究員 (40774592)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsカイコ / 長期保存 / ガラス化凍結 / CAS冷却 / 卵 / 生殖巣 / エリサン / シンジュサン / 凍結保存 / 生殖巣凍結 / 卵巣 / 精巣 / 胚子 / 精子
Outline of Final Research Achievements

The effectiveness of CAS freezer was studied from the point of using long-term preservation of silkworm. It was examined using fertilized egg and testis. In fertilized eggs, the effect of CAS freezer was a bit recognized, but it was judged not to contribute to practical application. There was no effect on testis freezing. Therefore, changing the method to achieve the purpose, freezing the embryo vitrified, and tested its effect. It was shown that silkworm embryos survive even after vitrified freezing, proceeding development and hatching. However, they could not eat mulberry leaves. They survived even after freezing, and the growth progressed until hatching, so it was judged that there was a possibility of achieving the purpose by examining the conditions in the future.
As second study, we evaluated the availability of the cryopreservation, which developed in the silkworm by using frozen ovary, for eri and ailanthus silkworms,and recognized positive results for both insect gonads.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] Long-term preservation of eri and ailanthus silkworms using frozen gonads.2017

    • Author(s)
      Fukumori H, Lee J, Fujii T, Kajiura Z, Banno Y.
    • Journal Title

      Cryobiology

      Volume: 77 Pages: 71-74

    • DOI

      10.1016/j.cryobiol.2017.05.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro culture and low temperature incubation tolerance of staged embryos of the silkworm, Bombyx mori2016

    • Author(s)
      H. Fukumori, T. Fujii and Y. Banno
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 85 Pages: 49-53

    • NAID

      130005421074

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] カイコ除殻卵の長期保存に向けたガラス化凍結の試み2018

    • Author(s)
      福森寿善, 伴野 豊
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カイコ卵巣凍結保存における魚類不凍タンパク質添加の影響2018

    • Author(s)
      吉永啓恵, 長崎紀代美, 江田将子, 福森寿善, 伴野 豊
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Crispr/Cas9による尿酸合成不全型油蚕(oya,ohi,oq)のモザイク解析2018

    • Author(s)
      藤井 告, 山本和典, 福森寿善, 伴野 豊
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 血中尿酸低下作用物質の探索(25) ―カミツレ Matricaria chamomilla L. 地上部の高活性フィトケミカルについて(II)―2018

    • Author(s)
      田中龍一郎, 森 龍介, 山口 昌之, 村田 咲樹, 坂崎 文俊, 伴野 豊, 秋澤 俊史
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カイコ凍結保存技術の高度化に向けたCASフリーザーの有効性の検証(予報)2016

    • Author(s)
      伴野豊・福森寿善・長崎紀代美・簑原由布子・藤井告
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      京都市京都工芸繊維大学
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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