Dying marker of host insects which induces the emergence of parasitoid wasp larvae

• Project/Area Number: 26660272
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Insect science
• Research Institution: Saga University
• Principal Investigator: Hayakawa Yoichi 佐賀大学, 農学部, 教授 (50164926)
• Research Collaborator: MATSUMOTO Hitoshi
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 昆虫 / 寄生蜂 / 脱出誘導 / 生理活性因子 / 宿主アワヨトウ / 脱出誘導因子 / 神経節 / 瀕死マーカー

Outline of Final Research Achievements

Female wasps of parasitoid Cotesia kariyai oviposit several tens of eggs into the host larvae of armyworm Pseudaletia separata at each parasitization and the wasp larvae emerge from their hosts almost simultaneously about 10 days after parasitization. We found that wasp larvae initiated their emergence from the posterior part of the host larvae tightly ligated at arround the middle of their abdomen shortly before the wasp emergence. Since the wasp emergence was found to be induced by a host hemolymph factor, we performed the purification and characterization of the factor. The structure of the purified active factor was determined by LC-MS/MS and NMR analyses. Furthermore, we confirmed that the active factor was secreted from hemocytes. The results of a series of incubation experiments suggested that the secretion of the active factor from hemocytes was induced by a novel factor secreted from brain-9th abdominal ganglion.

Research Products

• [Journal Article] Bacteria Endosymbiont, Wolbachia, Promotes Parasitism of Parasitoid Wasp Asobara japonica. (2015)
  • Author(s): Furihata, S., Hirata, M., Matsumoto, H., Hayakawa, Y.
  • Journal Title: Plos One Volume: 10 Issue: 10 Pages: e0140914-e0140914
  • DOI: 10.1371/journal.pone.0140914
  • Peer Reviewed / Open Access
• [Journal Article] Function of desiccate in gustatory sensilla of drosophila melanogaster. (2015)
  • Author(s): Kawano, T., Ryuda, M., Matsumoto, M., Ochiai, M., Oda, Y., Tanimura, T., Csikos, G., Moriya, M., Hayakawa, Y.
  • Journal Title: Sci. Rep. Volume: 27 Issue: 1 Pages: 17195-17195
  • DOI: 10.1038/srep17195
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification and functional characterization of a novel locust peptide belonging to the family of insect growth blocking peptides. (2015)
  • Author(s): Duressa,T.B, Boonen, K., Hayakawa, Y., Huybrechts, R.,
  • Journal Title: Peptides Volume: 74 Pages: 23-32
  • DOI: 10.1016/j.peptides.2015.09.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP. (2014)
  • Author(s): Tsuzuki, S. Matsumoto, H., Furihata, S., Ryuda, M., Tanaka, H., Sung, E-J., Bird, G.S., Zhou, Y., Shears, S.B., and Hayakawa, Y.,
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4628-4628
  • DOI: 10.1038/ncomms5628
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Immunoevasive protein (IEP)-containing surface layer covering polydnavirus particles is essential for viral infection. (2014)
  • Author(s): Furihata, S., Tanaka, K., Ryuda, M., Ochiai, M., Matsumoto, H., Csikos, G. and Hayakawa, Y.
  • Journal Title: J. Invertebr. Pathol. Volume: 115 Pages: 26-32
  • DOI: 10.1016/j.jip.2013.10.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gain of long tonic immobility behavioral trait causes the red flour beetle to reduce anti-stress capacity (2014)
  • Author(s): Kiyotake H, Matsumoto H, N akayama S, Sakai M, Miyata ke T, Ryuda M, and Hayakawa Y
  • Journal Title: Journal of Insect Physiology Volume: 60 Pages: 92-97
  • DOI: 10.1016/j.jinsphys.2013.11.008
  • Peer Reviewed
• [Journal Article] Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of the chalkbrood-infected colony after a pollination task in a greenhouse. (2014)
  • Author(s): Matsumoto, H., Nomura, S., Hayakawa, Y.
  • Journal Title: Applied Entomology and Zoology Volume: 49 Issue: 3 Pages: 395-402
  • DOI: 10.1007/s13355-014-0261-3
  • NAID: 40020169409
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stimulation of vitellogenin gene expression by permethrin in the brown planthopper, <i>Nilaparvata lugens</i> (Hemiptera: Auchenorrhyncha: Delphacidae) (2014)
  • Author(s): Khaeso, K., Matsumoto, H., Hayakawa, Y., Tojo, S.
  • Journal Title: Journal of Pesticide Science Volume: 39 Issue: 2 Pages: 98-104
  • DOI: 10.1584/jpestics.D13-052
  • NAID: 130004725729
  • ISSN: 1348-589X, 1349-0923
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 非寄生性線虫Caenorhabditis elegansによる昆虫血球の摂食 (2016)
  • Author(s): 小野雅哉・吉賀豊司・早川洋一
  • Organizer: 日本応用動物昆虫学会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2016-03-26
• [Book] Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research (2015)
  • Author(s): Yoichi Hayakawa (共著）
  • Total Pages: 674
  • Publisher: Academic Press
• [Patent(Industrial Property Rights)] ストレス耐性付与組成物、ストレス耐性付与方法、ストレス耐性体内増殖方法、及びストレス評価方法 (2016)
  • Inventor(s): 早川洋一
  • Industrial Property Rights Holder: 早川洋一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-03-23
• [Patent(Industrial Property Rights)] ストレス耐性付与組成物、ストレス耐性付与方法、ストレス耐性体内増殖方法、及びストレス評価方法 (2015)
  • Inventor(s): 早川洋一
  • Industrial Property Rights Holder: 早川洋一
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-059490
  • Filing Date: 2015-03-23