Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Outline of Final Research Achievements |
I tried CRISPR / Cas 9 in Nephotettix cincticeps (Hemiptera: Cicadellidae). RNA was injected into adult females, and genome modification in the next generation larvae was expected. It is the same method as parental RNAi, therefore, parental RNAi method was confirmed to be effective in N. cincticeps ( Arch Insect Biochem Physiol. 91:152-164.). CRISPR / Cas9 was performed on female adults using NcLac1S (a salivary gene) and NcLac2 (an epidermal laccase gene), and the genomic DNA of the next generation larvae was checked, but unfortunately no deletion or modification was observed.
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