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Does the plant have an ATG 5- or ATG 7- independent autophagy pathway?

Research Project

Project/Area Number 26660286
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionTohoku University

Principal Investigator

Ishida Hiroyuki  東北大学, 農学研究科, 准教授 (60312625)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsオートファジー / 葉緑体 / 老化 / ATG遺伝子 / 栄養リサイクル / 葉の老化 / オルガネラ
Outline of Final Research Achievements

The core ATG genes which are essential for autophagy in yeast is conserved in all eukaryotic organisms including plants. However, since Arabidopsis mutants which lack core ATG complete their life cycle, the existence of novel autophagy pathways which are different from the conventional-type autophagy. In this study, we examined the existence of ATG-independent novel autophagy pathway in Arabidopsis. We found that the number and size of chloroplasts per cell, which is known to be degraded by autophagy during leaf senescence, decreased in atg5 or atg7 mutants. We also tried to establish novel visualization methods for ATG-independent autophagy pathways in Arabidopsis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (17 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] From Arabidopsis to cereal crops: Conservation of chloroplast protein degradation by autophagy indicates its fundamental role in plant productivity.2015

    • Author(s)
      Izumi M, Hidema J, Ishida H
    • Journal Title

      Plant Signaling & Behavior

      Volume: 10 Issue: 11 Pages: e1101199-e1101199

    • DOI

      10.1080/15592324.2015.1101199

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation.2015

    • Author(s)
      Izumi M, Hidema J, Wada S, Kondo E, Kurusu T, Kuchitsu K, Makino A, Ishida H.
    • Journal Title

      Plant Physiology

      Volume: 169 Issue: 4 Pages: 1307-1320

    • DOI

      10.1104/pp.114.254078

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice2015

    • Author(s)
      Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
    • Journal Title

      Plant Physiology

      Volume: in press Issue: 1 Pages: 60-73

    • DOI

      10.1104/pp.15.00242

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Autophagy provides substrates to amino acid catabolic pathways as an adaptive response to sugar starvation in Arabidopsis2016

    • Author(s)
      Ishida, H.
    • Organizer
      The 17th International Congress on Photosynthesis Research
    • Place of Presentation
      MECC Maastricht, マーストリヒト、オランダ
    • Year and Date
      2016-08-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Autophagy of chloroplasts as an adaptive response to sugar starvation in Arabidopsis2016

    • Author(s)
      Ishida, H.
    • Organizer
      International Conference on Arabidopsis
    • Place of Presentation
      HICO, 慶州, 韓国
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chloroplast autophagy as an adaptive response to sugar starvation in Arabidopsis2016

    • Author(s)
      Hirota, T., Izumi, M., Wada, S., Makino, A., Ishida, H.
    • Organizer
      Gordon Research Conference ‘Mitochondria and Chloroplasts’
    • Place of Presentation
      Mt Snow Hotel, バーモント、USA
    • Year and Date
      2016-06-19
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に与える影響の解析2016

    • Author(s)
      横浜諒、和田慎也、菅野圭一、小島創一、山谷知行、牧野周、石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] イネの発芽過程におけるオートファジーの役割2015

    • Author(s)
      和田慎也、中村 萌、石田宏幸、牧野 周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナのRCBおよび葉緑体オートファジーにおけるATI(ATG8-interacting proteins)の役割について2015

    • Author(s)
      石田宏幸、西村 翼、泉 正範、Gad Galili、牧野 周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析2015

    • Author(s)
      和田慎也、林田泰和、泉 正範、来須孝光、花俣 繁、朽津和幸、牧野 周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] シロイヌナズナにおけるオートファジーが亜鉛欠乏時に果たす役割2015

    • Author(s)
      江口雅丈、吉本光希、木村和彦、泉 正範、和田慎也、牧野 周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation2015

    • Author(s)
      Masanori Izumi,Jun Hidema,Shinya Wada,Eri Kondo,Takamitsu Kurusu,Kazuyuki Kuchitsu,Amane Makino,Hiroyuki Ishida
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナの亜鉛欠乏条件下におけるオートファジーの役割2015

    • Author(s)
      江口雅丈,木村和彦,和田慎也,泉正範,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナの暗処理による炭素欠乏条件下でオートファジーは分岐鎖アミノ酸を代替呼吸基質として供給する2015

    • Author(s)
      弘田隆晃,泉正範,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおける オートファジーの役割の解析2015

    • Author(s)
      和田慎也,林田泰和, 泉正範,来須孝光,花俣繁,朽津和幸,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析2015

    • Author(s)
      横浜諒,和田慎也,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナにおける糖欠乏下でのオートファジーの役割の解析2014

    • Author(s)
      弘田隆晃・泉正範・牧野周・石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学、府中
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] シロイヌナズナにおける葉緑体オートファジーの機能解析2014

    • Author(s)
      泉正範・石田宏幸・牧野周・日出間純
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学、府中
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析2014

    • Author(s)
      和田慎也・横浜諒・石田宏幸・牧野周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学、府中
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] ロイヌナズナにおけるオートファジーの欠損が各栄養素の欠乏時の生存と成長に及ぼす影響の解析2014

    • Author(s)
      江口雅丈・泉正範・牧野周・石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学、府中
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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