Analysis of molecular mechanism of tolerance using tardigrade mitochondria
Project/Area Number |
26660288
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied molecular and cellular biology
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Research Institution | The University of Tokyo |
Principal Investigator |
Kunieda Takekazu 東京大学, 理学(系)研究科(研究院), 助教 (10463879)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ミトコンドリア / クマムシ / 乾燥耐性 / プロテオーム / 乾燥休眠 |
Outline of Final Research Achievements |
Some terrestrial tardigrades exhibit extreme desiccation-tolerance by entering a dehydrated state called anhydrobiosis. Molecular basis of anhydrobiosis remains largely unknown. In this study, we focused on the mitochondria of tardigrades as a simple model to elucidate the molecular basis of desiccation tolerance. Here, we identified two novel heat soluble proteins, which localize in mitochondria and transfection of these genes enhanced the tolerance of human cultured cells against osmotic stresses. Furthermore, we isolated a high purity mitochondrial fraction from tardigrades and comprehensively identified mitochondrial proteins using a shotgun proteomic approach. These proteins are served as good candidates involved in desiccation tolerance of tardigrade mitochondria.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Novel mitochondria-targeted heat-soluble proteins identified in the anhydrobiotic tardigrade improve osmotic tolerance of human cells.2015
Author(s)
Tanaka, S., Tanaka, J., Miwa Y., Horikawa, D.D., Katayama, T., Arakawa, K., Toyoda, A., Kubo, T., Kunieda, T.
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Journal Title
PLOS ONE
Volume: 10
Issue: 2
Pages: e0118272-e0118272
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Comparative Omic Analysis between anhydrobiotic tardigrade and desiccation-sensitive tardigrade2015
Author(s)
Takekazu Kunieda, Makiko Ito, Koyuki Kondo, Sae Tanaka, Takuma Hashimoto, Yohei Minakuchi, Hideki Noguchi, Toshiaki Katayama, Kazuharu Arakawa, Atsushi Toyoda,, Takeo Kubo, Asao Fujiyama
Organizer
13th International Symposium on Tardigrada
Place of Presentation
Modena, Italy
Year and Date
2015-06-23 – 2015-06-26
Related Report
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[Presentation] Comparative omic analysis between anhydrobiotic tardigrade and desiccation-sensitive tardigrade.2015
Author(s)
Kunieda T, Ito M, Kondo K, Minakuchi Y, Noguchi H, Tanaka S, Hashimoto T, Katayama T, Arakawa K, Toyoda A, Kubo T, Fujiyama A
Organizer
13th International Symposium on Tardigrada
Place of Presentation
Modena (Italy)
Year and Date
2015-06-23
Related Report
Int'l Joint Research
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[Presentation] A novel chromatin protein of radiation-resistant animal protects DNA and cells from radiation damages2015
Author(s)
Hashimoto T, Saito Y, Oyama M, Kozuka-Hata H, Enomoto A, Miyagawa K, Kuwahara H, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Fujiyama A, Kubo T, Kunieda T
Organizer
13th International Symposium on Tardigrada
Place of Presentation
Modena (Italy)
Year and Date
2015-06-23
Related Report
Int'l Joint Research
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[Presentation] A novel chromatin protein of radiation-resistant animal protects DNA and cells from radiation damages2015
Author(s)
Hashimoto T, Saito Y, Oyama M, Kozuka-Hata H, Enomoto A, Miyagawa K, Kuwahara H, Horikawa DD, Katayama T, Arakawa K, Toyoda A, Fujiyama A, Kubo T, Kunieda T
Organizer
15th International Congress of Radiation Research
Place of Presentation
国立京都国際会館(京都府京都市)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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