Molecular identity of large protein complexes with potential roles in cooperative regulation of different phases in plant cell cycle

• Project/Area Number: 26660290
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: Ito Masaki 名古屋大学, 生命農学研究科, 准教授 (10242851)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 転写因子複合体 / 細胞周期 / Myb転写因子 / E2F / シロイヌナズナ / DREAM複合体

Outline of Final Research Achievements

Proliferation of plant cells should be regulated spatially and temporally during plant organ growth. This regulation largely relies on the proper transcriptional regulation of the cohort of genes with cell cycle-related functions. Among them, two main classes of genes, G1/S- and G2/M-specific genes, are known to be regulated by families of transcription factors, called E2F and MYB3R, respectively. In this study, we first showed that MYB3R and E2F family proteins are present together in the same multi-protein complex in plants, which may be evolutionarily related to the protein complex, known as DREAM complex in animals such as human, fly, and worm. Unlike other organisms, plants specifically express multiple DREAM-like protein complexes that contain different members from E2F and MYB3R families and probably have distinct functions in the cell cycle.

Research Products

• [Int'l Joint Research] University of Tübingen(ドイツ)
• [Int'l Joint Research] Biological Research Centre(ハンガリー)
• [Int'l Joint Research] Aberystwyth University/University of London(英国)
• [Int'l Joint Research] Univ. Natural Resources Life Sci.(オーストリア)
• [Journal Article] Haspin kinase has multiple functions in the plant cell division regulatory network. (2016)
  • Author(s): Kozgunova, E., Suzuki, T., Ito, M., Higashiyama, T., Kurihara, D.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 4 Pages: 848-861
  • DOI: 10.1093/pcp/pcw030
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MYB3Rs, plant homologs of Myb oncoproteins, control cell cycle-regulated transcription and form DREAM-like complexes. (2015)
  • Author(s): Kobayashi, K., Suzuki, T., Iwata, E., Magyar, Z., Bogre, L., Ito, M.
  • Journal Title: Transcription Volume: 6 Issue: 5 Pages: 106-111
  • DOI: 10.1080/21541264.2015.1109746
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcriptional repression by MYB3R proteins regulates plant organ growth. (2015)
  • Author(s): Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettkó-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Bögre L, Ito M
  • Journal Title: EMBO Journal Volume: 34 Issue: 15 Pages: 1992-2007
  • DOI: 10.15252/embj.201490899
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reprogramming of plant cells induced by 6b oncoproteins from the plant pathogen Agrobacterium. (2015)
  • Author(s): Ito, M., Machida, Y.
  • Journal Title: J. Plant Res. Volume: 未定 Issue: 3 Pages: 423-435
  • DOI: 10.1007/s10265-014-0694-3
  • Peer Reviewed / Open Access
• [Journal Article] Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism. (2015)
  • Author(s): 丸山大輔、Ronny Völz、竹内英憲、森稔幸、井川智子、栗原大輔、河島友和、植田美那子、伊藤正樹、梅田正明、西川周一、Rita Groß-Hardt、東山哲也
  • Journal Title: Cell Volume: 未定 Issue: 4 Pages: 907-918
  • DOI: 10.1016/j.cell.2015.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Genetic interaction between G2/M phase-specific transcription factor MYB3R4 and MAPKKK ANP3 for execution of cytokinesis in Arabidopsis thaliana. (2015)
  • Author(s): Saito, T., Fujikawa, H., Haga, N., Suzuki, T., Machida, Y., Ito, M.
  • Journal Title: Plant Signal. Behav. Volume: 未定 Issue: 3 Pages: e990817-e990817
  • DOI: 10.4161/15592324.2014.990817
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rapid elimination of the persistent synergid through a cell-fusion for polytubey block. (2015)
  • Author(s): Maruyama, D., Völz, R., Takeuchi, H., Mori, T., Igawa, T., Kurihara, D., Kawashima, T., Ueda, M., Ito, M., Umeda, M., Nishikawa, S., Groß-Hardt, R., Higashiyama, T.
  • Journal Title: Cell Volume: 未定
  • Peer Reviewed
• [Journal Article] The carboxyl-terminal tail of the stalk of Arabidopsis NACK1/HINKEL kinesin is required for its localization to the cell plate formation site (2015)
  • Author(s): Sasabe, M., Ishibashi, N., Haruta, T., Minami, A., Kurihara, D., Higashiyama, T., Nishihama, R., Ito, M., and Machida, Y.
  • Journal Title: J. Plant Res. Volume: 128 Issue: 2 Pages: 327-336
  • DOI: 10.1007/s10265-014-0687-2
  • Peer Reviewed / Open Access
• [Presentation] 塩ストレス下での成長抑制に必須なシロイヌナスナMYB3R転写抑制因子 (2016)
  • Author(s): 奥村徹、鈴木孝征、東山哲也、伊藤正樹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-20
• [Presentation] シロイヌナズナを用いた後期促進複合体APC/Cの新奇標的因子の同定 (2016)
  • Author(s): 鈴木俊哉、桑田啓子、伊藤正樹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-20
• [Presentation] 多倍数体細胞を生じるイネ変異体の解析 (2015)
  • Author(s): 栂根美佳、栂根一夫、寺内良平、鈴木 孝征、東山哲也、長岐清孝、伊藤正樹
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-03
• [Presentation] 植物特異的なGRASファミリー転写因子による細胞分裂とDNA倍数性の制御 (2015)
  • Author(s): 鈴木俊哉、Christian Breuer、石田喬志、鈴木孝征、東山哲也、杉本慶子、伊藤正樹
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-02
• [Presentation] 発表標題塩ストレス下における植物の積極的な成長抑制 (2015)
  • Author(s): 奥村徹、伊藤正樹
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-07
  • Invited
• [Presentation] 葯壁タペート細胞で起こるDNA倍加のメカニズム (2015)
  • Author(s): 鈴木俊哉、浜村有希、東山哲也、石黒澄衛、伊藤正樹
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-07
• [Presentation] イネ体細胞のDNA倍数性に影響を与える遺伝子の解析 (2015)
  • Author(s): 栂根美佳、井上慎子、栂根一夫、寺内良平、永澤信洋、前川雅彦、伊藤正樹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-18
• [Presentation] GRASファミリー転写因子によるシロイヌナズナの細胞分裂と核内倍加の制御 (2015)
  • Author(s): 鈴木俊哉，Christian Breuer，石田喬志，鈴木孝征，東山哲也，杉本慶子，伊藤正樹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-18
• [Presentation] イネ体細胞のDNA倍数性に影響を与える遺伝子の解析 (2014)
  • Author(s): 栂根美佳、井上慎子、栂根一夫、寺内良平、永澤信洋、前川雅彦、伊藤正樹
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Presentation] 植物特異的なGRASファミリー転写因子による細胞周期の制御 (2014)
  • Author(s): 鈴木俊哉，Christian Breuer，石田喬志，鈴木孝征，東山哲也，杉本慶子，伊藤正樹
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Remarks] 伊藤研究グループ
  • URL: https://www.agr.nagoya-u.ac.jp/~bunka/ito_title%20page.html