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Exploring a novel mechanism regulating differentiation into beige adipocytes

Research Project

Project/Area Number 26660291
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyoto University

Principal Investigator

Kioka Noriyuki  京都大学, (連合)農学研究科(研究院), 准教授 (90234179)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords脂肪細胞 / コラーゲン / メカノバイオロジー / 褐色脂肪 / 細胞外マトリックス / 褐色脂肪細胞
Outline of Final Research Achievements

It has been revealed that white adipocytes can transdifferentiate into beige adipocytes, which consume lipids and generate heat. Stiffness of extracellular matrix affects cell fate and behaviors. In this research, we examined the effect of stiffness on the differentiation into beige adipocytes. We found that after induction of the differentiation, white adipocytes on gel substrates (soft) expressed more UCP1 mRNA, beige adipocyte maker, than those on plastic dishes (very rigid). This supports the hypothesis that stiffness affects the differentiation of white adipocytes into beige adipocytes.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (2 results)

  • [Journal Article] Interaction of the vinculin proline-rich linker region with vinexin α in sensing extracellular matrix stiffness2014

    • Author(s)
      Hiroshi Yamashita, Takafumi Ichikawa, Daisuke Matsuyama, Yasuhisa Kimura, Kazumitsu Ueda, Susan W. Craig, Ichiro Harada, and Noriyuki Kioka
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 1875-86

    • DOI

      10.1242/jcs.133645

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation2014

    • Author(s)
      Tomiyama, L., Sezaki, T., Matsuo, M., Ueda, K. and Kioka, N.
    • Journal Title

      Oncogene

      Volume: 33 Issue: 9 Pages: 1141-1149

    • DOI

      10.1038/onc.2014.31

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] ビネキシンαとホスファチジン酸の相互作用解析2016

    • Author(s)
      伊藤有亮、市川尚文、木村泰久、植田和光、木岡紀幸
    • Organizer
      2016年(平成28年度)日本農芸化学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Vinculin regulates the ECM-stiffness dependent adipogenesis and the nuclear localization of YAP/TAZ2015

    • Author(s)
      黒田 美都, 植田 和光, 木岡 紀幸
    • Organizer
      iCeMS International Symposium "Hierarchical Dynamics in Soft Materials and Biological Matter"
    • Place of Presentation
      京都
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of the vinculin with vinexin α in ECM-stiffness directed cell differentiation and migration2015

    • Author(s)
      木岡紀幸
    • Organizer
      「上皮管腔組織形成」 第2回国際シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2015-08-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Membrane microdomain and mechanosensor for stiffness of extracellular matrix(ECM)2015

    • Author(s)
      木岡紀幸
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ビネキシンファミリータンパク質の接着斑を介したメカノセンシング機能の比較解析2015

    • Author(s)
      市川尚文、喜多真弘、木村泰久、植田和光、木岡紀幸
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞外基質の硬さを感知するメカノセンサー2015

    • Author(s)
      木岡紀幸
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation2014

    • Author(s)
      Lucia Tomiyama, Takuhito Sezaki, Michinori Matsuo, Kazumitsu Ueda, and Noriyuki Kioka
    • Organizer
      2014 Annual Meeting of The American Society for Cell Biology
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2014-12-06 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Distribution of vinculin to lipid rafts is involved in sensing ECM stiffness2014

    • Author(s)
      Ayaka Nagasato, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda, and Noriyuki Kioka
    • Organizer
      2014 Annual Meeting of The American Society for Cell Biology
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2014-12-06 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞外マトリックスの硬さに応じた脂肪細胞分化にビンキュリンが与える影響2014

    • Author(s)
      黒田美都、和田洋樹、植田和光、木岡紀幸
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 接着斑タンパク質ビンキュリンは間葉系幹細胞のECMの硬さ依存的な脂肪細胞分化に必要である2014

    • Author(s)
      黒田美都、和田洋樹、植田和光、木岡紀幸
    • Organizer
      第19回日本肥満学会アディポサイエンスシンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Focal adhesion protein vinculin is necessary for regulating the ECM-stiffness dependent adipocyte differentiation of mesenchymal stem cells2014

    • Author(s)
      黒田美都、和田洋樹、植田和光、木岡紀幸
    • Organizer
      第66回日本細胞生物学会
    • Place of Presentation
      奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Interaction of the vinculin proline-rich linker region with vinexin alpha in sensing extracellular matrix stiffness2014

    • Author(s)
      Takafumi Ichikawa, Hiroshi Yamashita, Daisuke Matsuyama, Yasuhisa Kimura, Kazumitsu Ueda, Susan W. Craig, Ichiro Harada, and Noriyuki Kioka
    • Organizer
      International symposium on Mechanobiology 2014
    • Place of Presentation
      岡山
    • Year and Date
      2014-05-20 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Remarks] 京都大学農学研究科応用生命科学専攻細胞生化学研究室

    • URL

      http://www.biochemistry.kais.kyoto-u.ac.jp/

    • Related Report
      2015 Annual Research Report
  • [Remarks]

    • URL

      http://www.biochemistry.kais.kyoto-u.ac.jp/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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