| Project/Area Number |
26660292
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKIHIRO Takashi 島根大学生, 物資源科学部, 助教 (40508941)
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| Research Collaborator |
TAKEUCHI Takashi
ABE Takuma
Smirnoff Nicholas
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アスコルビン酸輸送体 / シロイヌナズナ / 非光化学消光 / 葉緑体 / アスコルビン酸輸送変異体 / アスコルビン酸 / 輸送機構 / 酵母発現ライブラリー |
|---|
| Outline of Final Research Achievements |
In this study, we performed a comprehensive screening to isolate a
novel Arabidopsis mutant which show abnormal recovery for non-photochemical quenching after feeding of ascorbate. We finally obtained at least two novel possibilities by following reasons; they showed low NPQ values after supplementation of ascorbate in the growth medium, and their plastids did not show any histochemical staining by silver nitrate treatment. We also confirmed that the mutants showed no substitution at least in the known causal genes including pht4;4 and npq mutants, strongly suggesting that the obtained mutants are our objectives.
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