| Project/Area Number |
26670015
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIYAMA Naoyuki 京都大学, 大学院薬学研究科, 准教授 (50545704)
WAKABAYASHI Masaki 京都大学, 大学院薬学研究科, 助教 (70552024)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | プロテオミクス / LC-MS / プロテオーム / 質量分析 |
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| Outline of Final Research Achievements |
We aimed to develop a deep proteomics analytical system to identify and quantify complete human proteome expressed in human cells. We developed ultrahigh resolution LC-MS systems using meter-scale monolithic silica capillary columns in combined with meter-scale HILIC columns for fractionating or slicing samples. As a result, when using the slicing mode, the number of identified proteins increased by 46%, compared with the conventional method, Further, using the fractionation method, we successfully identified 10,000 human proteins with 200-fold sensitivity and 6-times throughput.
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