| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
For analyzing the function of microRNA in the regulation of innate immune responses against intracellular microbes, such as intracellular bacteria or viruses, we performed next generation sequencing of miRNA in Drosophila. As a result, we identified 5 miRNA and 2 miRNA, expression of which is increased to Listeria or Vesicular stomatitis virus infection, respectively. miR-14, present in common of the both above, functions in autophagic cell death. We further analyzed the fuction of Ref(2)P, an adaptor of the selective autophagy, to show that it is essential against virus infection, and when viruses are orally infected to Drosophila adults, Ref(2)P makes an aggregate in intestinal epithelial cells. These results suggest the importance of Ref(2)P aggregate formation in the innate immune responses against viruses, and its regulation could be a novel target of virus regulation.
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