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Development of cancer stem cells driven by microenvironment

Research Project

Project/Area Number 26670023
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionJuntendo University (2015-2017)
St. Luke's International University (2014)

Principal Investigator

IRIMURA Tatsuro  順天堂大学, 医学部, 特任教授 (80092146)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsがん幹細胞 / ZnT1 / モノクローナル抗体 / 大腸がん / 乳がん / フローサイトメトリー / 非対称分裂 / ZnT1、、非対称分裂 / モのクロ-ナル抗体
Outline of Final Research Achievements

Cancer stem cells are important subjects to understand the treatment resistance, latency and recurrence of cancer and to improve therapy. However, it was not easy to identify them in histological and cytological context. The mechanism how they give rise should be investigated with appropriate surface markers, which was not available. We have recently prepared a monoclonal antibody against ZnT1, a cancer stem cell surface marker candidate discovered by genetic analysis. Our specific aim was to visualize the developmental state of cancer stem cells by the use of the antibody. However, after careful investigations conducted under the present project, we concluded that the anti-ZnT1 antibody positive cell population was not identical to cancer stem cell populations identified by other methods. The expression status of this molecule did not correlate with the treatment resistance of cancer. However, we found that the developed monoclonal antibody is useful as a tool to analyze ZnT1.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (44 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (18 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 17 results,  Acknowledgement Compliant: 7 results,  Open Access: 10 results) Presentation (22 results) (of which Int'l Joint Research: 7 results,  Invited: 12 results) Book (2 results)

  • [Int'l Joint Research] Jagiellonian University(Poland)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] UT-MD Anderson Cancer Center(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Microbial metabolites and derivatives targeted at inflammation and bone diseases therapy: chemistry, biological activity and pharmacology.2018

    • Author(s)
      Adachi H, Nakae K, Sakamoto S, Nosaka C, Atsumi S, Shibuya M, Higashi N, Nakajima M, Irimura T, Nishimura Y.
    • Journal Title

      J Antibiot (Tokyo).

      Volume: 71 Issue: 1 Pages: 60-71

    • DOI

      10.1038/ja.2017.138

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Critical Domain of Ebolavirus Envelope Glycoprotein Determines Glycoform and Infectivity2018

    • Author(s)
      Fujihira H, Usami K, Matsuno K, Takeuchi H, Denda-Nagai K, Furukawa JI, Shinohara Y, Takada A, Kawaoka Y, Irimura T
    • Journal Title

      Sci Rep

      Volume: 8 Issue: 1 Pages: 5495-5495

    • DOI

      10.1038/s41598-018-23357-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteasome inhibitor loaded micelles enhance antitumor activity through macrophage reprograming by NF-κB inhibition"2017

    • Author(s)
      Hailiang Wu, Anqi Tao, John D. Martin, Sabina Quader, Xueying Liu, Kei Takahashi, Louise Hespel, Yutaka Miura, Yoshihiro Hayakawa, Tatsuro Irimura, Horacio Cabral, Kazunori Kataoka
    • Journal Title

      J. Pharm. Sci,

      Volume: - Issue: 9 Pages: 30217-30224

    • DOI

      10.1016/j.xphs.2017.03.031

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] IL-17A-producing CD30(+) Vδ1 T cells drive inflammation-induced cancer progression.2016

    • Author(s)
      Kimura Y, Nagai N, Tsunekawa N, Sato-Matsushita M, Yoshimoto T, Cua DJ, Iwakura Y, Yagita H, Okada F, Tahara H, Saiki I, Irimura T, Hayakawa Y.
    • Journal Title

      Cancer Sci.

      Volume: 107 Issue: 9 Pages: 1206-14

    • DOI

      10.1111/cas.13005

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An iminosugar-based heparanase inhibitor heparastatin (SF4) suppresses infiltration of neutrophils and monocytes into inflamed dorsal air pouches.2016

    • Author(s)
      Sue M, Higashi N, Shida H, Kogane Y, Nishimura Y, Adachi H, Kolaczkowska E, Kepka M, Nakajima M, Irimura T.
    • Journal Title

      Int Immunopharmacol

      Volume: 35 Pages: 15-21

    • DOI

      10.1016/j.intimp.2016.03.017

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Report on the use of nonclinical studies in the regulatory evaluation of oncology drugs2016

    • Author(s)
      Hayakawa Y, Kawada M, Nishikawa H, Ochiya T, Saya H, Seimiya H, Yao Y, Hayashi M, Kai C, Matsuda A, Naoe T, Ohtsu A, Okazaki T, Saji H, Sata M, Sugimura H, Sugiyama Y, Toi M, Irimura T
    • Journal Title

      Cancer Sci

      Volume: 107(2) Issue: 2 Pages: 189-202

    • DOI

      10.1111/cas.12857

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Heparanase augments inflammatory chemokine production from colorectal carcinoma cell lines.2016

    • Author(s)
      Tsunekawa N, Higashi N, Kogane Y, Waki M, Shida H, Nishimura Y, Adachi H, Nakajima M, Irimura T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 469 Issue: 4 Pages: 878-883

    • DOI

      10.1016/j.bbrc.2015.12.074

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mammary tissue microenvironment determines T cell-dependent breast cancer-associated inflammation.2015

    • Author(s)
      Takahashi K, Nagai N, Ogura K, Tsuneyama K, Saiki I, Irimura T, Hayakawa Y.
    • Journal Title

      Cancer Sci.

      Volume: 106 Issue: 7 Pages: 867-874

    • DOI

      10.1111/cas.12685

    • NAID

      120006389612

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Viwatthanasittiphong C, Srivatanakul P, Miwa M, Shoda J, Narimatsu H. Lectin Microarray-Based Sero-Biomarker Verification Targeting Aberrant O-Linked Glycosylation on Mucin 1.2015

    • Author(s)
      Matsuda A, Kuno A, Nakagawa T, Ikehara Y, Irimura T, Yamamoto M, Nakanuma Y, Miyoshi E, Nakamori S, Nakanishi H.
    • Journal Title

      Anal Chem.

      Volume: 87(14) Issue: 14 Pages: 7274-7281

    • DOI

      10.1021/acs.analchem.5b01329

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] POMGNT1 Is Glycosylated by Mucin-Type <i>O</i>-Glycans2015

    • Author(s)
      Xin X, Akasaka-Manya K, Manya H, Furukawa J, Kuwahara N, Okada K, Tsumoto H, Higashi N, Kato R, Shinohara Y, Irimura T, Endo T.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 38 Issue: 9 Pages: 1389-1394

    • DOI

      10.1248/bpb.b15-00415

    • NAID

      130005096979

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 免疫系とバイオマテリアル:総論2015

    • Author(s)
      入村達郎、伝田香里
    • Journal Title

      Biomaterials

      Volume: 33(2) Pages: 8-15

    • Related Report
      2015 Research-status Report
    • Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mammary tissue microenvironment determines T cell-dependent breast cancer-associated inflammation.2015

    • Author(s)
      Takahashi K, Nagai N, Ogura K, Tsuneyama K, Saiki I, Irimura T, Hayakawa Y.
    • Journal Title

      Cancer Sci

      Volume: 15

    • NAID

      120006389612

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The macrophage galactose-type lectin can function as an attachment and entry receptor for influenza virus.2014

    • Author(s)
      Ng WC, Liong S, Tate MD, Irimura T, Denda-Nagai K, Brooks AG, Londrigan SL, Reading PC.
    • Journal Title

      Jornal of Virology

      Volume: 88(3) Issue: 3 Pages: 1659-72

    • DOI

      10.1128/jvi.02014-13

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heparanase-mediated cleavage of macromolecular heparin accelerates release of granular components of mast cells from extracellular matrices.2014

    • Author(s)
      Higashi N, Waki M, Sue M, Kogane Y, Shida H, Tsunekawa N, Hasan A, Sato T, Kitahara A, Kasaoka T, Hayakawa Y, Nakajima M, Irimura T.
    • Journal Title

      Biochemical Jornal

      Volume: 458(2) Issue: 2 Pages: 291-9

    • DOI

      10.1042/bj20131463

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of neogenin-1 increases cell proliferation and motility in gastric cancer.2014

    • Author(s)
      Kim SJ, Wang YG, Lee HW, Kang HG, La SH, Choi IJ, Irimura T, Ro JY, Bresalier RS, Chun KH.
    • Journal Title

      Oncotarget

      Volume: 5(10) Pages: 3386-3398

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interaction of Muc4 and ErbB2 in a transgenic mouse model of gallbladder carcinoma: potential pathobiological implications.2014

    • Author(s)
      Miyahara N, Shoda J, Kawamoto T, Ishida H, Ueda T, Akimoto Y, Kawakami H, Irimura T.
    • Journal Title

      Oncol Rep

      Volume: 32(5) Issue: 5 Pages: 1796-1802

    • DOI

      10.3892/or.2014.3443

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of Hirsutine as an anti-metastatic phytochemical by targeting NF-kB activation2014

    • Author(s)
      Chenghua Lou, Kei Takahashi, Tatsuro Irimura, Ikuo Saiki and Yoshihiro Hayakawa
    • Journal Title

      International Journal of Oncology

      Volume: 45 Issue: 5 Pages: 2085-2091

    • DOI

      10.3892/ijo.2014.2624

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CXCL16 suppresses liver metastasis of colorectal cancer by promoting TNF-α-induced apoptosis by tumor-associated macrophages.2014

    • Author(s)
      Kee JY, Ito A, Hojo S, Hashimoto I, Igarashi Y, Tsuneyama K, Tsukada K, Irimura T, Shibahara N, Takasaki I, Inujima A, Nakayama T, Yoshie O, Sakurai H, Saiki I, Koizumi K.
    • Journal Title

      Cancer Sci

      Volume: 14 Pages: 949-949

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 糖鎖付加MUC1を利用したがんワクチンのための基礎的研究:MGLの関与2018

    • Author(s)
      伝田香里、入村達郎
    • Organizer
      糖鎖免疫研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ムチンの機能と疾患2018

    • Author(s)
      入村達郎
    • Organizer
      御茶ノ水アレルギー研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ムチン21とその糖鎖:がんによる免疫抑制とアポトーシスの抑制に関わるムチン2017

    • Author(s)
      入村達郎
    • Organizer
      糖鎖免疫シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2017-01-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Comparison on immune response after immunization with glycosylated MUC1 between C57BL/6 mice and MUC1 transgenic mice2017

    • Author(s)
      Denda-Nagai K, Namba A, Noji M, Irimura T
    • Organizer
      Mucins in Health and Diseases, 14th Internantional Workshop on Carcinoma-Associated Mucins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of Mucin 1 glycoprotein expression in estrogen receptor-positive primary breast cancer as a predictive marker of neoadjuvant chemotherapy effects and patient outcomes2017

    • Author(s)
      Matsuzawa M, Horimoto Y, Okazaki M, Suzuki M, Fujihira H, Noji M, Denda-Nagai K, Mogushi K, Nakai K, Saito M, Irimura T
    • Organizer
      Mucins in Health and Diseases, 14th Internantional Workshop on Carcinoma-Associated Mucins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Difference in humoral immune response after immunization with glycosylated MUC1 between C57BL/6 mice and MUC1 transgenic mice2017

    • Author(s)
      Kaori Denda-Nagai, Tatsuro Irimura
    • Organizer
      第46回日本免疫学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Future of glycobiologics2016

    • Author(s)
      Tatsuro Irimura
    • Organizer
      Frontier 2016-UT/EPFL Joint Symposium
    • Place of Presentation
      Lausanne
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] MUC1 with truncated O-glycans induces humoral immune responses and suppresses the carcinoma growth and metastasis2015

    • Author(s)
      Kaori Denda-Nagai, Tatsuro Irimura
    • Organizer
      第44回日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2015-11-20
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] C-Type Lectins onDendritic Cell and Macrophages Regulate Infection and Immuity2015

    • Author(s)
      Irimura Tatsuro
    • Organizer
      LMU-UT Symposium
    • Place of Presentation
      Munich, Germany
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] エボラウイルス-MGL 相互作用の謎2015

    • Author(s)
      藤平陽彦、宇佐美克明、伝田香里、古川潤一、篠原康郎、松野啓太、高田礼人、河岡義裕、入村 達郎
    • Organizer
      糖鎖免疫シンポジウム
    • Place of Presentation
      東京医科歯科大学
    • Year and Date
      2015-08-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] がん悪性化進展を促す炎症性免疫応答の維持機構におけるIL-17の重要性2015

    • Author(s)
      早川芳弘、永井直、斉木育夫、入村達郎
    • Organizer
      第24回日本がん転移学会
    • Place of Presentation
      シティプラザ大阪
    • Year and Date
      2015-07-24
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] MGL(CD301)in infection and immunity2015

    • Author(s)
      Irimura Tatsuro
    • Organizer
      International Workshop on Mucins in Health and diseases
    • Place of Presentation
      Cambridge, UK
    • Year and Date
      2015-07-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 薬科学に未来はあるか2015

    • Author(s)
      入村達郎
    • Organizer
      日本薬剤会第30回記念大会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 糖鎖付加MUC1に対する免疫応答による癌の増殖と転移の制御2015

    • Author(s)
      難波愛理、伝田香里、野地美樹、入村達郎
    • Organizer
      第135日本薬学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Heparanase-mediated cleavage of macromolecular heparin accelerates release of granular components of mouse peritoneal cell-derived mast cells.2014

    • Author(s)
      東伸昭,入村達郎
    • Organizer
      第43回日本免疫学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] マクロファージガラクトース型C型レクチン(MGL/CD301)による炎症と免疫の制御2014

    • Author(s)
      伝田香里、入村達郎
    • Organizer
      第12回糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Identification of Hirsutine as an anti-metastatic phytochemical by targeting NF-κB activation.2014

    • Author(s)
      Lou C, Takahashi K, Irimura T, Saiki I, Hayakawa Y.
    • Organizer
      第73回日本癌学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] A unique dermal dendritic cell subset that skews the immune response toward Th2.2014

    • Author(s)
      Irimura T.
    • Organizer
      MGH and U-Tokyo Joint Symposium on Frontiers in Biomedical Engineering
    • Place of Presentation
      Cambridge, MA, USA
    • Year and Date
      2014-09-24 – 2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Public private partnership in Japanese pharmaceutical affairs.2014

    • Author(s)
      Irimura T.
    • Organizer
      FIP Congress
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] マスト細胞顆粒内ヘパリンの切断は細胞外マトリックスからの生理活性物質の放出を促進する2014

    • Author(s)
      東伸昭,中島元夫、入村達郎
    • Organizer
      第33回日本糖質学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-08-10 – 2014-08-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Vd1T細胞を起点とする自然炎症はがん悪性化を促進する2014

    • Author(s)
      早川芳弘、済木育夫、入村達郎
    • Organizer
      第23回がん転移学会学術集会
    • Place of Presentation
      金沢
    • Year and Date
      2014-07-10 – 2014-07-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Requirement of CD4T cells for establishing inflammatory tumor microenvironment in 4T1breast cancer model.2014

    • Author(s)
      Takahashi K, Nagai N, Saiki I, Irimura T, Hayakawa Y.
    • Organizer
      The 22nd International Symposium on Molecular Cell Biology of Macrophages.
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      2014-06-02 – 2014-06-03
    • Related Report
      2014 Research-status Report
  • [Book] 感染と免疫 第4版2017

    • Author(s)
      J.Playfair、G.Bancroft、入村 達郎、伝田 香里、加藤 健太郎、佐藤 佳代子、築地 信
    • Total Pages
      264
    • Publisher
      東京化学同人
    • ISBN
      9784807908974
    • Related Report
      2017 Annual Research Report
  • [Book] C-Type Lectin Receptors in Immunity2016

    • Author(s)
      Kaori Denda-Nagai and Tatsuro Irimura
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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