| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
G protein coupled receptors (GPCRs) are one of the remarkable targets for the design of therapeutic drugs. Many of screening methods have been developed to identify effective agents for GPCR signaling. We previously developed a bioluminescence measuring system to detect the interaction of GPCR with β-arrestin based on split luciferase fragment complementation. During the observation of bioluminescence, there is one of the hypotheses that GPCR signals transmit among cells.
To demonstrate the hypothesis, we developed new bioluminescence imaging technique for the temporal monitoring of GPCR-β-arrestin interaction in living cells. Simultaneous imaging of GPCR activity and cell movements indicated that GPCR activity linked the stage of cell proliferation.
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