Utilization of indigenous bacteria to produce recombinant proteins in animal body

• Project/Area Number: 26670025
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: KAITO CHIKARA 東京大学, 薬学研究科(研究院), 准教授 (60420238)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ロイヤルゼリー / 常在細菌 / フタホシコオロギ / カイコ / カイコガ / クワガタムシ / 腸管 / 昆虫 / 体サイズ

Outline of Final Research Achievements

This study identified bacterial species that exist in insect guts and examined its activity of genetic transformation and reattachment to insect guts. We isolated several bacterial species from stag beetles and the two-spotted cricket. We found that one of the bacterial species could be transformed with plasmid DNA and reattached to cricket gut.
In addition, this study searched biological molecules that increase insect body size. We found that administration of royal jelly to silkworms or crickets increases their body sizes. The result suggests that biological molecules in royal jelly have an activity to increase insect body size.

Research Products

• [Journal Article] No effect of body size on the frequency of calling and courtship song in the two-spotted cricket, Gryllus bimaculatus (2016)
  • Author(s): A Miyashita, H Kizaki, K Sekimizu, C Kaito
  • Journal Title: PloS one Volume: 11 Issue: 1 Pages: e0146999-e0146999
  • DOI: 10.1371/journal.pone.0146999
  • URL: https://pure.teikyo.jp/en/publications/2e897fc9-f6c5-4582-bf84-65f6bc0c42d3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Primed immune responses triggered by ingested bacteria lead to systemic infection tolerance in silkworms (2015)
  • Author(s): Miyashita, A. Takahashi, S. Ishii, K. Sekimizu, K. Kaito, C.
  • Journal Title: PLoS One Volume: 10 Issue: 6 Pages: e0130486-e0130486
  • DOI: 10.1371/journal.pone.0130486
  • URL: https://pure.teikyo.jp/en/publications/48ac0a3e-410b-4555-83c0-3b368a698b71
  • Peer Reviewed / Open Access
• [Journal Article] Antibiotic-producing bacteria from stag beetle mycangia (2015)
  • Author(s): Miyashita A, Hirai Y, Sekimizu K, Kaito C.
  • Journal Title: Drug Discoveries & Therapeutics Volume: 9 Issue: 1 Pages: 33-37
  • DOI: 10.5582/ddt.2015.01000
  • NAID: 130005054806
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Microbe-host interactions revealed using the silkworm infection model (2016)
  • Author(s): 垣内 力
  • Organizer: 第89回日本細菌学会総会 シンポジウム
  • Place of Presentation: 大阪国際交流センター、大阪府大阪市
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] 昆虫モデルを利用した細菌ー宿主相互作用の解明 (2015)
  • Author(s): 垣内 力
  • Organizer: 生物の共生進化を考える（昆虫共生酵母研究会主催シンポジウム）
  • Place of Presentation: 島根大学、島根県松江市
  • Year and Date: 2015-06-02
  • Invited