Genes that enable high-efficiency gene disruption in human cells

• Project/Area Number: 26670031
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Yokohama City University
• Principal Investigator: Adachi Noritaka 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (30264675)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム改変 / ジーンターゲティング / Nalm-6

Outline of Final Research Achievements

In this research project, we have identified several human genes that increase the efficiency of gene targeting by homologous recombination. We have shown for the first time that an enzyme with cytidine deaminase activity can enhance gene targeting in NHEJ-null cells.

Research Products

• [Journal Article] Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification (2016)
  • Author(s): Saito S, Adachi N.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 39 Issue: 1 Pages: 25-32
  • DOI: 10.1248/bpb.b15-00701
  • NAID: 130005116505
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection. (2015)
  • Author(s): Saito S, Ura K, Kodama M, Adachi N.
  • Journal Title: BMC Res Notes. Volume: 8 Issue: 1 Pages: 278-278
  • DOI: 10.1186/s13104-015-1241-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role for Artemis nuclease in the repair of radiation-induced DNA double strand breaks by alternative end joining. (2015)
  • Author(s): Moscariello M, Wieloch R, Kurosawa A, Li F, Adachi N, Mladenov E, Iliakis G.
  • Journal Title: DNA Repair
  • Peer Reviewed
• [Journal Article] Role of Auf1 in elimination of oxidatively damaged messenger RNA in human cells. (2015)
  • Author(s): Ishii T, Hayakawa H, Sekiguchi T, Adachi N, Sekiguchi M.
  • Journal Title: Free Radic Biol Med. Volume: 79 Pages: 109-116
  • DOI: 10.1016/j.freeradbiomed.2014.11.018
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of the role of homology arms in gene-targeting vectors in human cells. (2014)
  • Author(s): Ishii A, Kurosawa A, Saito S, Adachi N.
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: e108236-e108236
  • DOI: 10.1371/journal.pone.0108236
  • Peer Reviewed / Open Access / Acknowledgement Compliant