Screening of anti-tuberculosis substances produced by microorganism

• Project/Area Number: 26670049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Kitasato University
• Principal Investigator: Miura Hiromi 北里大学, 感染制御科学府, 助教 (20462252)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Atsuko 北里大学, 感染制御科学府, 講師 (20300759) ; NAKASHIMA Takuji 北里大学, 北里生命科学研究所, 特任准教授 (40526216)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Yōko 北里大学, 感染制御科学府, 研究員 (80197186) ; SHIOMI Kazuro 北里大学, 感染制御科学府, 教授 (40235502) ; IWATSUKI Masato 北里大学, 感染制御科学府, 講師 (70353464)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 抗結核菌物質 / 放線菌 / 微生物培養抽出液 / 二次代謝産物 / 抗結核菌作用 / 糸状菌 / 抗結核菌

Outline of Final Research Achievements

To discover anti-tuberclosis drugs with a new mechanisms of action, we invented simple screening system that is targeted to biosynthesis pathway of diaminopimelate (DAP). We screened approximately 9,600 culture broths of actinomycete and fungi. As a result, we found anti-mycobacterium substance produced by Streptomyces sp. K12-0214. This substance is water-soluble, irreversibly inactivated under basic condition. Anti-microbial spectrum of this material was different from that of existing drug isoniadide and rifampicin.

Research Products

• [Presentation] 放線菌が生産する抗結核菌物質の探索 (2015)
  • Author(s): 三浦 広美, 岩月 正人, 稲橋 佑起, 松本 厚子, 中島 琢自, 塩見 和朗, 高橋 洋子, 大村 智
  • Organizer: 2015年度 日本放線菌学会大会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2015-09-07