| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Atropisomer dinapinones A1 and A2 (DPA1 and DPA2) were isolated from a culture of Talaromyces pinophilus FKI-3864. Monapinone coupling enzyme (MCE) which dimerizes naphthopyranone monapinone A (MPA) to regioselectively synthesize DPA (a mixture of atropisomers DPA1 and DPA2) was purified from T. pinophilus FKI-3864. In this study, we established an efficient procedure to purify MCE easily and heterologous expression system of MCE gene in Escherichia coli, Aspergilluys oryzae and Saccharomyces cervisiae. Furthermore, to identify regioselective coupling reaction of MCE, enzymatic analysis and substrate specificity were investigated.
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