Construction of bispecific antibodies from the camelid antibody VHHs via in-cell protein trans-splicing
| Project/Area Number |
26670051
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Yamagata University |
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Principal Investigator |
Makabe Koki 山形大学, 理工学研究科, 准教授 (20508072)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 蛋白質工学 / 抗体工学 / 蛋白質トランススプライシング / 二重特異性抗体 / 重鎖抗体 / 抗体 / 蛋白質ライゲーション / インテイン |
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| Outline of Final Research Achievements |
Production of various combinations of bispecific antibodies (bsAbs) is important for evaluating the next generation antibody drugs. In spite of its importance, it requires several gene-engineering steps. Here, we report an alternative approach for bsAb construction to reduce the gene manipulation steps. We used a method to create bsAbs in vivo through protein trans-splicing. For proof-of-concept of this method, we show the construction of a bsAb with anti-epidermal growth factor receptor variable domain of heavy chain antibody (VHH) and anti-green fluorescent protein (GFP) VHH. We successfully constructed the bsAb using this method and the resulting bsAb has the bispecific activity.
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Report
(3 results)
Research Products
(5 results)
