Development of CpG island binding molecule based on artificial dinucleotide
| Project/Area Number |
26670056
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 核酸医薬 / 核酸化学 / 分子認識 / 人工核酸 / 核酸誘導体 / CpGアイランド / ジヌクレオチドユニット |
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| Outline of Final Research Achievements |
In this study, we tried to create novel artificial molecules capable of binding to CpG islands deeply involved in the epigenetic regulation mechanism of gene expression. Focusing on the G/GC base triplet structure of natural type triplex DNA formation, an artificial nucleic acid analogue having an aromatic ring at the 2-position of guanosine was designed as a recognition molecule. We have succeeded in chemical synthesis of the target compound. After incorporation of them into oligonucleotide using an automated DNA synthesizer, we evaluated the interaction with duplex DNA and synthesized oligonucleotides. We found that the artificial nucleic acid containing benzene ring could recognize the GC base pair with high stability and selectivity by comparing the association constant of the natural type guanosine. Moreover, we succeeded in finding that it has recognition ability if only one is contained regardless of where it is incorporated in the oligonucleotide.
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Report
(4 results)
Research Products
(9 results)
