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Rapid monitoring of harmful bacteria in aquatic environment by in liquid-fluorescence in situ hybridization

Research Project

Project/Area Number 26670062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionOsaka Prefectural Institute of Public Health (2016)
Osaka University (2014-2015)

Principal Investigator

Yamaguchi Nobuyasu  大阪府立公衆衛生研究所, 衛生化学部, 総括研究員 (20252702)

Co-Investigator(Kenkyū-buntansha) 一條 知昭  大阪大学, 薬学研究科, 助教 (20513899)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsオンサイト・モニタリング / 危害微生物 / 水環境 / マイクロ流路デバイス / 蛍光 in situ ハイブリダイゼーション
Outline of Final Research Achievements

Assessing microbiological quality assurance by monitoring bacteria in aquatic environment as well as food and drink is necessary for our healthy life, and the change in number of harmful bacteria should be determined as quickly as possible. Thus, “real-time” and “on-site” microbiological methods are required. In this study, we investigated “in liquid-fluorescence in situ hybridization” technique, which perform whole procedure in liquid to detect harmful microbes selectively without complex procedure. Target bacterial cells can be counted within 5 h by the combination of this new technique and our original counting system, microfluidic system.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system2017

    • Author(s)
      N. Yamaguchi, Y. Tokunaga, S. Goto, Y. Fujii, F. Banno, A. Edagawa
    • Journal Title

      Scientific Reports

      Volume: -

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Development of liq-FISH method and selective detection of harmful bacteria with microfluidic system2016

    • Author(s)
      N. Yamaguchi, S. Goto, M. Nasu.
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      Palais des Congres de Montreal(モントリオール,カナダ)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 溶液中FISH法の開発およびマイクロ流路システムを用いた危害細菌の特異的検出2016

    • Author(s)
      山口 進康,後藤 聡子,那須 正夫
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 溶液中FISH法の開発およびマイクロ流路システムを用いた危害細菌の特異的検出2015

    • Author(s)
      山口 進康,後藤 聡子,那須 正夫
    • Organizer
      フォーラム2015:衛生薬学・環境トキシコロジー
    • Place of Presentation
      神戸
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 宇宙居住環境におけるon-site微生物モニタリングを目指したマイクロ流路システムの開発2014

    • Author(s)
      山口 進康,藤井 雄大,那須 正夫
    • Organizer
      環境微生物系合同大会2014
    • Place of Presentation
      アクトシティ浜松コングレスセンター(浜松)
    • Year and Date
      2014-10-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 宇宙居住環境におけるon-site微生物モニタリングを目指したマイクロ流路システムの開発2014

    • Author(s)
      山口 進康,藤井 雄大,那須 正夫
    • Organizer
      フォーラム2014:衛生薬学・環境トキシコロジー
    • Place of Presentation
      つくば国際会議場(筑波)
    • Year and Date
      2014-09-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Real-time and on-site monitoring of bacterial cells in aquatic environments by portable microfluidic system2014

    • Author(s)
      N. Yamaguchi, Y. Fujii, T. Tanizawa, F. Banno and M. Nasu.
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      COEX Center (Seoul Korea)
    • Year and Date
      2014-08-25
    • Related Report
      2014 Research-status Report
  • [Presentation] On-site and real-time monitoring for bacterial cells in freshwater with microfluidic system2014

    • Author(s)
      N. Yamaguchi, Y. Fujii, T. Tanizawa, F. Banno and M. Nasu.
    • Organizer
      American Society for Microbiology 2014 General Meeting
    • Place of Presentation
      Boston Convention & Exhibition Center (Boston, USA)
    • Year and Date
      2014-05-18
    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪大学大学院薬学研究科 衛生・微生物学分野 webページ:宇宙居住と微生物

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b017/kenkyu/kenkyu3.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 大阪大学大学院薬学研究科 衛生・微生物学分野webページ

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b017/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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