| Project/Area Number |
26670067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUIJI Makoto 星薬科大学, 薬学部, 准教授 (90302611)
OKU Teruaki 星薬科大学, 薬学部, 助教 (20409361)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞接着 / 白血球浸潤 / 細菌分泌タンパク質 / マトリックス分解酵素 / 黄色ブドウ球菌 / シアル酸 / セレクチン / SSL / MMP / マトリックスメタロプロテイナーゼ |
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| Outline of Final Research Achievements |
1) We observed that staphylococcal superantigen-like protein 5 (SSL5) bound to matrix metalloproteinase-9 (MMP-9) and inhibited its enzymatic activity. The enzyme plays a crucial role in the accumulation of leukocytes to infection sites. When MMP-9 was treated with sialidase or peptide:N-glycanase, the interaction between MMP-9 and SSL5 was weakened, suggesting that carbohydrate chains containing sialic acid residues were involved in the binding of the two molecules.
2) We screened the culture supernatants of various bacterial species (A. hydrophila, E. coli, L. monocytogenes, M. bovis, S. aureus) for the proteins bound to the P-selectin/Fc fusion protein, and detected several different proteins in these bacterial culture supernatants. We are examining the effects of these proteins on the selectin-dependent leukocyte adhesion.
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