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Suppression of host defense system by bacterial "cell adhesion-perturbing proteins"

Research Project

Project/Area Number 26670067
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionHoshi University

Principal Investigator

Tsuji Tsutomu  星薬科大学, 薬学部, 教授 (00143503)

Co-Investigator(Renkei-kenkyūsha) TSUIJI Makoto  星薬科大学, 薬学部, 准教授 (90302611)
OKU Teruaki  星薬科大学, 薬学部, 助教 (20409361)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞接着 / 白血球浸潤 / 細菌分泌タンパク質 / マトリックス分解酵素 / 黄色ブドウ球菌 / シアル酸 / セレクチン / SSL / MMP / マトリックスメタロプロテイナーゼ
Outline of Final Research Achievements

1) We observed that staphylococcal superantigen-like protein 5 (SSL5) bound to matrix metalloproteinase-9 (MMP-9) and inhibited its enzymatic activity. The enzyme plays a crucial role in the accumulation of leukocytes to infection sites. When MMP-9 was treated with sialidase or peptide:N-glycanase, the interaction between MMP-9 and SSL5 was weakened, suggesting that carbohydrate chains containing sialic acid residues were involved in the binding of the two molecules.
2) We screened the culture supernatants of various bacterial species (A. hydrophila, E. coli, L. monocytogenes, M. bovis, S. aureus) for the proteins bound to the P-selectin/Fc fusion protein, and detected several different proteins in these bacterial culture supernatants. We are examining the effects of these proteins on the selectin-dependent leukocyte adhesion.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (6 results)

  • [Journal Article] Development of splice variant-specific monoclonal antibodies against human alpha3 integrin2016

    • Author(s)
      Oku T, Ando Y, Ogura M, Tsuji T
    • Journal Title

      Monoclon Antib Immunodiagn Immunother

      Volume: 35 Issue: 1 Pages: 12-17

    • DOI

      10.1089/mab.2015.0053

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Platelets attenuate production of cytokines and nitric oxide by macrophages in response to bacterial endotoxin2015

    • Author(s)
      Ando Y, Oku T, Tsuji T
    • Journal Title

      Platelets

      Volume: 27 Issue: 4 Pages: 1-7

    • DOI

      10.3109/09537104.2015.1103369

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of liposomal nanoconstructs targeting P-selectin (CD62P)-expressing cells by using a sulfated derivative of sialic acid2014

    • Author(s)
      Itoh S, Kawano K, Takeshita K, Maitani Y, Tsuji T
    • Journal Title

      Pharm Res

      Volume: (in press) Issue: 10 Pages: 2868-2875

    • DOI

      10.1007/s11095-014-1383-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 黄色ブドウ球菌由来SSL5とMMP-9の結合における糖鎖の役割2016

    • Author(s)
      栗坂知里,奥 輝明,辻 勉
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微生物成分に対するマクロファージの応答に及ぼす血小板由来因子の影響2016

    • Author(s)
      安藤祐介,奥 輝明,辻 勉
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板はマクロファージのエンドトキシン応答を抑制する2015

    • Author(s)
      安藤祐介,奥 輝明,辻 勉
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板由来可溶性因子はマクロファージのLPSに対する応答を抑制する2015

    • Author(s)
      安藤祐介,奥 輝明,辻 勉
    • Organizer
      第16回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2015-06-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] SSL-5とmatrix metalloproteinase 9の結合2015

    • Author(s)
      栗坂知里,奥 輝明,伊藤佐生智,辻 勉
    • Organizer
      第16回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2015-06-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硫酸化シアル酸誘導体NMSO3によるP - セレクチン依存的な細胞接着の制御とDDSへの応用2014

    • Author(s)
      伊藤佐生智,小代智紀,工藤早苗,川野久美,米谷芳枝,辻 勉
    • Organizer
      第15回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      名古屋市立大学
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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