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Study on the exosomes as a biomarker to estimate in vivo function of human blood-brain barrier

Research Project

Project/Area Number 26670072
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Medical pharmacy
Research InstitutionChiba University

Principal Investigator

Chiba Kan  千葉大学, 薬学研究科(研究院), 教授 (40159033)

Co-Investigator(Kenkyū-buntansha) FURIHATA Tomomi  千葉大学, 大学院薬学研究院, 助教 (80401008)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsバイオマーカー / エキソソーム / 血液脳関門
Outline of Final Research Achievements

The blood-brain barrier (BBB) is composed of brain microvascular enodothelial cells, astrocytes, and pericytes, and plays important roles in restricting penetration of various drugs into the brain. Therefore, it is important to develop a biomarker that allows us to estimate in vivo BBB functions. In this study, we aimed to clarify whether exosomes (Ex) derived from human brain microvascular endothelial cells (HBMEC) contains similar mRNA cargo to that of the parental cells. We developed a normal BBB model, as well as an impaired BBB model, where VE-cadherin mRNA level was significantly decreased. The Ex derived from each model were collected and their mRNA patterns were examined. The results show that the VE-cadherin mRNA expression level in the Ex derived from the impaired BBB model cells was clearly lower than that from the normal BBB model cells. Thus, our results suggest that the Ex derived from HBMEC may have a potential to be a biomarker for estimating in vivo BBB functions.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] ヒト血液脳関門コリントランスポーターの同定とその血液脳関門機能における役割の解明2015

    • Author(s)
      鈴木翔太、降幡知巳、伊藤涼、周徐嘉、稲津正人、千葉寛
    • Organizer
      第10回トランスポーター研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Identification and functional characterization of human blood-brain barrier choline transporter in HBMEC/ciβ, an immortalized human brain microvascular endothelial cell line.2015

    • Author(s)
      Ito R, Furihata T, Suzuki S, Chiba K.
    • Organizer
      11th Cerebrovascular biology
    • Place of Presentation
      Paris, France
    • Year and Date
      2015-06-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2017-05-10  

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