Development of a novel model of alveolar type I cells for the study on drug transport and toxicity in the lung
| Project/Area Number |
26670078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
TAKANO MIKIHISA 広島大学, 医歯薬保健学研究院(薬), 教授 (20211336)
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| Co-Investigator(Renkei-kenkyūsha) |
YUMOTO RYOKO 広島大学, 大学院医歯薬保健学研究院, 准教授 (70379915)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 薬学 / 肺胞上皮細胞 / Ⅰ型細胞 / モデル細胞開発 / 分化転換 |
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| Outline of Final Research Achievements |
The distal air spaces of the lungs are lined with a continuous epithelium comprising two types of alveolar epithelial cells, type I and type II. At present, no good cell line having type I cell-like phenotype is available. In alveolar epithelial cells, P-glycoprotein (P-gp) is selectively expressed in type I cells but not in type II cells. Using a limited dilution method, we successfully isolated an A549 clone (A549/P-gp) highly expressing P-gp as well as type I cell marker mRNAs, while P-gp expression in native A549 cells was negligible. Using A549/P-gp cells, the effect of cigarette smoke extract (CSE) on P-gp function in the lung alveoli was examined, and we found that CSE had inhibitory effect on P-gp function. A549/P-gp cells would be useful as a novel model of alveolar type I cells.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] Cisplatin resistance in human lung cancer cells is linked with dysregulation of cell cycle associated proteins.2015
Author(s)
Horibe S, Matsuda A, Tanahashi T, Inoue J, Kawauchi S, Mizuno S, Ueno M, Takahashi K, Maeda Y, Maegouchi T, Murakami Y, Yumoto R, Nagai J, Takano M.
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Journal Title
Life Sciences
Volume: １２４
Pages: 31-40
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts.2014
Author(s)
Takano, M., Kakizoe, S., Kawami M., Nagai J., Patanasethanont, D., Sripanidkulchai B., Yumoto, R.
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Journal Title
Pharmazie
Volume: 69
Pages: 823-828
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Expression and function of a peptide transporter PEPT2 in alveolar epithelial cells in culture2014
Author(s)
Yumoto, R., Sugimoto, N., Sasaki, Y., Yamamoto, C., Nagai, J., Ehrhardt, C., Takano, M.
Organizer
The 5th FIP Pharmaceutical Sciences World Congress 2014
Place of Presentation
Melbourne (Australia)
Year and Date
2014-04-13 – 2014-04-16
Related Report
