| Project/Area Number |
26670078
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
TAKANO MIKIHISA 広島大学, 医歯薬保健学研究院(薬), 教授 (20211336)
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| Co-Investigator(Renkei-kenkyūsha) |
YUMOTO RYOKO 広島大学, 大学院医歯薬保健学研究院, 准教授 (70379915)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 薬学 / 肺胞上皮細胞 / Ⅰ型細胞 / モデル細胞開発 / 分化転換 |
|---|
| Outline of Final Research Achievements |
The distal air spaces of the lungs are lined with a continuous epithelium comprising two types of alveolar epithelial cells, type I and type II. At present, no good cell line having type I cell-like phenotype is available. In alveolar epithelial cells, P-glycoprotein (P-gp) is selectively expressed in type I cells but not in type II cells. Using a limited dilution method, we successfully isolated an A549 clone (A549/P-gp) highly expressing P-gp as well as type I cell marker mRNAs, while P-gp expression in native A549 cells was negligible. Using A549/P-gp cells, the effect of cigarette smoke extract (CSE) on P-gp function in the lung alveoli was examined, and we found that CSE had inhibitory effect on P-gp function. A549/P-gp cells would be useful as a novel model of alveolar type I cells.
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