Establishment of quantification system for human urinary LC3 as a noninvasive pharmacological evaluation system of mTOR inhibitor
| Project/Area Number |
26670081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
KAJIWARA Moto
TAJIMA Soichiro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バイオマーカー / mTOR阻害薬 / オートファジー / 薬剤性腎障害 / 慢性腎臓病 / 腎臓移植 |
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| Outline of Final Research Achievements |
Autophagy is an adaptation to starvation that escapes cell death through the reuse of amino acids, and is also activated by the inhibition of mTOR (mammalian target of rapamycin) activity. The increase of LC3 expression is well known as an indicator of autophagy. In the present study, a measurement system capable of quantifying the leakage of LC3 in human urine has been successfully established. LC3 in the urine is derived from cells constituting the kidney, and is an epoch-making technology that can noninvasively monitor the state of autophagy in the kidney. It is expected to be applied in the future as a state of the transplanted kidney tissue undergoing ischemia reperfusion injury and a pharmacological effect in human kidneys when mTOR inhibitors those induces autophagy is administered.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Molecular Markers of Tubulointerstitial Fibrosis and Tubular Cell Damage in Patients with Chronic Kidney Disease2015
Author(s)
Nakagawa S, Nishihara K, Miyata H, Shinke H, Tomita E, Kajiwara M, Matsubara T, Iehara N, Igarashi Y, Yamada H, Fukatsu A, Yanagita M, Matsubara K & Masuda S
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Journal Title
PLoS ONE
Volume: 10
Pages: 1-14
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Urinary neutrophil gelatinase-associated lipocalin: a useful biomarker for tacrolimus-induced acute kidney injury in liver transplant patients2014
Author(s)
Tsuchimoto A*, Shinke H*, Uesugi M, Kikuchi M, Hashimoto E, Sato T, Ogura Y, Hata K, Fujimoto Y, Kaido T, Kishimoto J, Yanagita M, Matsubara K, Uemoto S, Masuda S.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Impact of cytochrome P450 3A5 polymorphism in graft livers on the frequency of acute cellular rejection in living-donor liver transplantation.2014
Author(s)
Uesugi M, Kikuchi M, Shinke H, Omura T, Yonezawa A, Matsubara K, Fujimoto Y, Okamoto S, Kaido T, Uemoto S, Masuda S.
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Journal Title
Pharmacogenet Genomics
Volume: 24
Pages: 356-366
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] mTOR阻害薬の可能性2014
Author(s)
増田智先
Organizer
第39回西日本薬剤学研究会
Place of Presentation
九州地区国立大学九重共同研修所
Year and Date
2014-08-29
Related Report
Invited
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