Comprehensive screening of histone modifying enzyme

• Project/Area Number: 26670085
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Keiko 東北大学, 医学(系)研究科(研究院), 教授 (60294972)
• Co-Investigator(Kenkyū-buntansha): FUNAYAMA Ryo 東北大学, 大学院医学系研究科, 助教 (20452295)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: CRISPR/Cas9 / メチル化酵素 / ES細胞 / 3T3-L1

Outline of Final Research Achievements

In the nucleus, core histones, which genomic DNA wrap around, create an environment of genome DNA. Post-translational modifications of histone are thought to regulate the shape of existence and function of genomic DNA. This means the significance to elucidate the regulatory mechanisms of post-translational modifications of histone.
In this project, the final goal was set as the generation of cell group which is consist of cells with mutation of various enzymes of modification, and the examination of change of constituent cells after long-term culture or induction of differentiation. Effective protocol of introduction of mutation was determined for several cell lines.

Research Products

• [Journal Article] CRL4 E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases (2015)
  • Author(s): Nakagawa, T., Lv, L., Nakagawa, M., Yu, Y., Yu, C., D'Alessio, A. C., Nakayama, K., Fan, H., Chen, X. & Xiong, Y.
  • Journal Title: Mol Cell Volume: 57 Issue: 2 Pages: 247-260
  • DOI: 10.1016/j.molcel.2014.12.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of acquired mutations by whole-genome sequencing in GATA-2 deficiency evolving into myelodysplasia and acute leukemia. (2014)
  • Author(s): Fujiwara, T., Fukuhara, N., Funayama, R., Nariai, N., Kamata, M., Nagashima, T., Kojima, K., Onishi, Y., Sasahara, Y., Ishizawa, K., Nagasaki, M., Nakayama, K. & Harigae, H
  • Journal Title: Ann Hematol Volume: 93 Issue: 9 Pages: 1515-1522
  • DOI: 10.1007/s00277-014-2090-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] HCV infection enhances Th17 commitment, which could affect the pathogenesis of autoimmune diseases (2014)
  • Author(s): Kondo Y, Ninomiya M, Kimura O, Machida K, Funayama R, Nagashima T, Kobayashi K, Kakazu E, Kato T, Nakayama K, Lai MM, Shimosegawa T.
  • Journal Title: PLoS One Volume: 9(6) Issue: 6 Pages: e98521-e98521
  • DOI: 10.1371/journal.pone.0098521
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] GNE myopathy associated with congenital thrombocytopenia: a report of two siblings. (2014)
  • Author(s): Izumi R, Niihori T, Suzuki N, Sasahara Y, Nishiyama A, Nishiyama S, Endo K, Kato M, Warita H, Konno H, Tateyama M, Nagashima T, Funayama R, Nakayama K, Kure S, Matsubara Y, Aoki Y, Aoki M.
  • Journal Title: Neuromuscul. Disord. Volume: 24(12) Issue: 12 Pages: 1068-1072
  • DOI: 10.1016/j.nmd.2014.07.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] マウスES細胞におけるgemininの細胞周期と分化に対する制御 (2014)
  • Author(s): 福本恵美子, Kundu, Lena Rani, 佐藤聡一郎, 細金正樹 & 中山啓子
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] H3K27me3修飾パターン形成過程の時系列解析 (2014)
  • Author(s): 細金正樹, 舟山亮, 城田松之 & 中山啓子
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] 精子形成におけるE3ユビキチンリガーゼβ-TrCPの新規基質の同定と解析 (2014)
  • Author(s): 久志瞭, 中川直, 中野星児, 遠藤尚博 & 中山啓子
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] TFIID complex changes accompany epithelial-mesenchymal transition (EMT) (2014)
  • Author(s): Nakagawa, Tadashi & Nakayama, Keiko
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25
• [Presentation] がん遺伝子による転写変化とエピゲノム変化 (2014)
  • Author(s): 中山啓子
  • Organizer: 第23回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎大学医学部良順会館（長崎県長崎市）
  • Year and Date: 2014-11-04
  • Invited
• [Remarks] がん医学コアセンター 細胞増殖制御分野
  • URL: http://www.devgen.med.tohoku.ac.jp/index.html