| Project/Area Number |
26670088
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
Katoh Hiroto 東京医科歯科大学, 難治疾患研究所, 助教 (60446549)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 細胞分化 / 次世代シーケンス / shRNAライブラリ / 機能ゲノミクススクリーニング |
|---|
| Outline of Final Research Achievements |
The aim of this study was to develop fundamental research protocols for a novel high-throughput screening method in order to identify any defined RNAi combinations to control cell lineages via novel RNAi-based reprograming strategies. Particularly, to develop such a screening method, I combined whole-genomic shRNA lentivirus library and next-generation sequence technique. I developed and fine-tuned several complexed experimental protocols related to Lentivirus production, Lentivirus infection, cell culture, cell-sorting or cell-picking, and next-generation sequencing. I could establish some of the fundamental research protocols to be utilized in the high-throughput screening to search for defined RNAi combinations to induce lineage reprograming.
|
