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A novel screening method to identify any defined RNAi combinations to induce cell reprogramming.

Research Project

Project/Area Number 26670088
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Katoh Hiroto  東京医科歯科大学, 難治疾患研究所, 助教 (60446549)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞分化 / 次世代シーケンス / shRNAライブラリ / 機能ゲノミクススクリーニング
Outline of Final Research Achievements

The aim of this study was to develop fundamental research protocols for a novel high-throughput screening method in order to identify any defined RNAi combinations to control cell lineages via novel RNAi-based reprograming strategies. Particularly, to develop such a screening method, I combined whole-genomic shRNA lentivirus library and next-generation sequence technique. I developed and fine-tuned several complexed experimental protocols related to Lentivirus production, Lentivirus infection, cell culture, cell-sorting or cell-picking, and next-generation sequencing. I could establish some of the fundamental research protocols to be utilized in the high-throughput screening to search for defined RNAi combinations to induce lineage reprograming.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2015

All Presentation (2 results)

  • [Presentation] 次世代シーケンス技術と網羅的shRNAライブラリーを利用した新規胃癌治療標的遺伝子スクリーニング法の樹立2015

    • Author(s)
      藤橋未希、加藤洋人、佐藤玲子、鈴木良平、貴志一樹、河村大輔、石川俊平
    • Organizer
      日本人類遺伝学会第60回大会
    • Place of Presentation
      京王プラザホテル、東京都新宿区
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 網羅的shRNAライブラリーと次世代シーケンス技術を利用した新規胃癌治療標的遺伝子の探索2015

    • Author(s)
      藤橋未希、加藤洋人、佐藤玲子、鈴木良平、貴志一樹、河村大輔、石川俊平
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋国際会議場、名古屋市
    • Year and Date
      2015-04-20
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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