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Analysis of novel actin filament binding protein on the synaptic biology and autism.

Research Project

Project/Area Number 26670089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionUniversity of Fukui

Principal Investigator

Kuroda Kazuki  福井大学, 医学部, 助教 (60557966)

Co-Investigator(Kenkyū-buntansha) Sato Makoto  大阪大学, 連合小児発達学研究科, 教授 (10222019)
Yagi Hideshi  兵庫医科大学, 医学部, 教授 (10303372)
Xie Min-Jue  福井大学, 医学部, 助教 (40444210)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsmyosin / actin / spine / hippocampus / シナプス / スパイン / アクチン / 自閉症 / ミオシン / NMDA受容体 / 海馬 / 大脳皮質
Outline of Final Research Achievements

Non-muscle myosin IIb plays a major role for regulation of actin dynamics in the dendritic spines. However, how myosin IIb directly alters cytoskeletal dynamics through ATPase-driven contraction of actin networks and how myosin IIb function is regulated during the spine maturation are still poorly understood. We found that FRM was a binding partner of myosin IIb and was expressed in the hippocampal and neocortical neurons. We next examined the effects of altered endogenous FRM expression in cultured hippocampal neurons, the knockdown of FRM expression induced the spine length shortening and changed the ratio of cell surface and total expressing NMDA receptor. Recently, we generated FRM conditional knockout mice for understanding of the roles of FRM in the hippocampus and the cerebral cortex. When FRM conditional knockout mice crossed with Emx1-Cre mice, these FRM mutant mice showed the anxiety-like behavior compared with the control mice.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Filamin A interacting protein plays a role in proper positioning of callosal projection neurons in the cortex2016

    • Author(s)
      Yagi H, Oka Y, Komada M, Xie MJ, Noguchi K, Sato M.
    • Journal Title

      Neurosci Lett

      Volume: 612 Pages: 18-24

    • DOI

      10.1016/j.neulet.2015.11.049

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy.2014

    • Author(s)
      Chunhui Jiang, Yefei Wen, K.Kuroda, Kevin Hannon, Michael A. Rudnicki, Shihuan Kuang
    • Journal Title

      Dis Model Mech.

      Volume: 7 Pages: 997-1004

    • DOI

      10.1242/dmm.015917

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Filamin A-interacting protein (FILIP) is a region-specific modulator of myosin 2b and controls spine morphology and NMDA receptor accumulation.2014

    • Author(s)
      Hideshi Yagi,Takashi Nagano,Min-Jue Xie,Hiroshi Ikeda,Kazuki Kuroda,Munekazu Komada,Tokuichi Iguchi,Rahman M. Tariqur,Soichi Morikubo,Koichi Noguchi,Kazuyuki Murase,Masaru Okabe& Makoto Sato
    • Journal Title

      Scientific reports

      Volume: 4 Issue: 1 Pages: 6353-6353

    • DOI

      10.1038/srep06353

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] FILIP-related molecule binds to NMDA receptor and controls spine maturation and synaptic function of the hippocampal neuron.2015

    • Author(s)
      Kazuki Kuroda, Hideshi Yagi, Min-Jue Xie, Yugo Fukazawa, Yuichiro Oka, Tokuichi Iguchi, Makoto Sato
    • Organizer
      Society for Neuroscience 2015
    • Place of Presentation
      シカゴ、米国
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海馬神経細胞におけるFILIP関連分子の機能解析2015

    • Author(s)
      黒田一樹、謝敏カク、深澤有吾、猪口徳一、岡雄一郎、八木秀司、佐藤真
    • Organizer
      日本解剖学会第75回中部支部学術集会
    • Place of Presentation
      永平寺町、福井
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] FILIP-related molecule binds to NMDA receptor and controls spine maturation and synaptic function of the hippocampal neuron2015

    • Author(s)
      Kazuki Kuroda, Hideshi Yagi, Min-Jue Xie, Minoru Omi, Yuichiro Oka, Tokuichi Iguchi, Makoto Sato
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸市、兵庫県
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 神経細胞のスパインにおけるNMDA受容体と結合するFILIP関連分子の機能解析2015

    • Author(s)
      黒田一樹, 八木秀司, 謝敏カク, 尾身実, 猪口徳一, 岡雄一郎, 佐藤真
    • Organizer
      第120回日本解剖学会、第92回日本生理学会大会
    • Place of Presentation
      神戸市、神戸国際会議場・展示場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Phldb2はLTD誘導後のシナプスでのAMPA受容体のエンドサイトーシスを制御する2014

    • Author(s)
      謝敏カク, 八木秀司, 猪口徳一, 岡雄一郎, 黒田一樹, 柚崎通介, 松田信爾, 石川保幸, 佐藤真
    • Organizer
      第36回日本生物学的精神医学会、第57回日本神経化学会大会
    • Place of Presentation
      奈良市、奈良県文化会館・新公会堂
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Research-status Report
  • [Presentation] Phldb2は樹状突起スパインの成熟および可塑性を制御する2014

    • Author(s)
      謝敏カク, 八木秀司, 猪口徳一, 黒田一樹, 岡雄一郎, 柚崎通介, 松田信爾, 石川保幸, 佐藤真
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      横浜市、パシフィコ横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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