| Project/Area Number |
26670091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Ikegami Koji 浜松医科大学, 医学部, 准教授 (20399687)
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| Co-Investigator(Renkei-kenkyūsha) |
SETOU MITSUTOSHI 浜松医科大学, 医学部, 教授 (20302664)
SUGIURA YUKI 慶応大学, 医学部, 講師 (30590202)
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| Research Collaborator |
KONNO ALU 日本学術振興会, 特別研究員 PD
YAMAZAKI FUMIYOSHI 浜松医科大学, 特任助教
VU Thi Hang 浜松医科大学, 大学院生
MORI YASUKO 浜松医科大学, JRリサーチアシスタント
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 質量分析 / 翻訳後修飾 / 細胞骨格 / 微小管 / チューブリン / 中枢神経系 / 直接検出 / イメージング / ラベルフリー |
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| Outline of Final Research Achievements |
We aimed to establish a novle technique to detect directly protein post-translational modifications, which occur on many proteins constucting living organisms, without labeling. To this end, we tried to seek out optimal conditions that acheive the direct detection of post-translational modifications by means of mass spectrometry, targeting proteins and post-translational modifications abundant in neurons of the nervous system. We succeded in establishing a novel and simple method by which post-translational modifications in tissue samples are detected directly, though failing to establish the new technique for the direct detection of post-translational modifications on tissue specimens without extraction of proteins from tissues. Our acheivement was published as a chapter of a book written in English, which will be relased all ovre the world.
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