Cell lineage analysis of mouse embryos

• Project/Area Number: 26670092
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: The University of Tokushima
• Principal Investigator: TAKEMOTO Tatsuya 徳島大学, 先端酵素学研究所(オープンイノベ), 助教 (30443899)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞系譜 / 原腸陥入 / 細胞分化

Outline of Final Research Achievements

In our previous study, we have showed that the neural plate and paraxial mesoderm originate from their common precursors, axial stem cells. This challenges the textbook view where neural plate is generated from ectoderm by separating epidermis. In this study, we studied cell lineage of mouse embryos, especially focusing on the stage of E6-E7.

Research Products

• [Journal Article] Axial level-dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate. (2016)
  • Author(s): Kondoh H, Takada S, Takemoto T.
  • Journal Title: Development, Growth & Differentiation. Volume: 58 Issue: 5 Pages: 427-427
  • DOI: 10.1111/dgd.12295
  • Peer Reviewed
• [Journal Article] R26-WntVis reporter mice showing graded response to Wnt signal levels. (2016)
  • Author(s): Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
  • Journal Title: Genes Cells. Volume: 印刷中 Issue: 6 Pages: 661-669
  • DOI: 10.1111/gtc.12364
  • NAID: 120006306641
  • Peer Reviewed / Open Access
• [Journal Article] Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. (2016)
  • Author(s): Hashimoto M, Yamashita Y, Takemoto T.
  • Journal Title: Developmental Biology Volume: 418 Issue: 1 Pages: 1-9
  • DOI: 10.1016/j.ydbio.2016.07.017
  • Peer Reviewed
• [Journal Article] Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. (2015)
  • Author(s): Hashimoto M. and Takemoto T.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11315-11315
  • DOI: 10.1038/srep11315
  • NAID: 120006875680
  • Peer Reviewed / Open Access
• [Presentation] High-throughput production of mutant mice by electroporation of CRISPR/Cas9 system (2015)
  • Author(s): Takemoto T and Hashimoto M
  • Organizer: Mouse Molecular Genetics 2015
  • Place of Presentation: Cambridge (UK)
  • Year and Date: 2015-09-19
  • Int'l Joint Research
• [Presentation] High-throughput production of mutant mice by electroporation of CRISPR/Cas9 system (2015)
  • Author(s): Takemoto T and Hashimoto M
  • Organizer: 48th Annual Meeting of the Japanese Society of Developmental Biology
  • Place of Presentation: つくば国際会議場（茨城県・つくば市）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Remarks] 徳島大学先端酵素学研究所発生生物学分野ホームページ
  • URL: http://www.fujii.tokushima-u.ac.jp/embryology/
• [Remarks] 徳島大学藤井節郎記念医科学センター初期発生研究分野ホームページ
  • URL: http://www.fujii.tokushima-u.ac.jp/embryology/