A genetic approach for the understanding of the integrity of Blood-Brain Barrier in Drosophila.
Project/Area Number |
26670095
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General anatomy (including histology/embryology)
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Research Institution | Keio University |
Principal Investigator |
KANDA Hiroshi 慶應義塾大学, 医学部(信濃町), 講師 (60508597)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 血液脳関門 / 遺伝学 / ショウジョウバエ / BBB |
Outline of Final Research Achievements |
We have elucidated the molecular mechanisms of genes whose function is required for the establishment and/or the maintenance of the integrity of Blood-Brain Barrier (BBB). Drosophila melanogaster (fruit fly) was used as the model organism. We have mainly focused on three different types of genes. Firstly, we analyzed a gene whose function is required for the establishment of the barrier. The knockdown of this gene did not affect the viability of the cells, however, it resulted in the hypoplasia of the BBB. Secondly, we analyzed a gene whose loss-of-function mutant animal emerged from a pupa with an almost intact BBB, however, its integrity decreased age-dependent manner. Finally, the knockdown of a gene resulted in not only the attenuation of the barrier integrity, but it also induced an increase in the proliferation of neural stem cells. This result strongly suggested a novel physiological significance of the BBB.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Musashi mediates translational repression of the Drosophila hypoxia inducible factor.2016
Author(s)
Bertolin AP, Katz MJ, Yano M, Pozzi B, Acevedo JM, Blanco-Obregon D, Gandara L, Sorianello E, Kanda H, Okano H, Srebrow A, Wappner P.
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Journal Title
Nucleic Acids Research
Volume: 19
Issue: 16
Pages: 7555-67
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Musashi mediates translational repression of the Hypoxia Inducible Factor.2016
Author(s)
Bertolin AP, Katz MJ, Acevedo J, Pozzi B, Blanco-Obregon D, Yano M, Gandara L, Kanda H, Okano H, Srebrow A, and Wappner P.
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Journal Title
Nucl. Acid Res.
Volume: 未定
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 血液脳関門2016
Author(s)
菅田浩司
Organizer
グローバルサイエンスキャンパス事業
Place of Presentation
慶應義塾大学日吉キャンパス(神奈川県横浜市)
Related Report
Invited
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